Thermo Scientific™ Lysozyme

The Thermo Scientific™ Lysozyme is a highly purified enzyme characterized by its ability to efficiently lyse bacterial cell walls, improving the extraction of proteins and nucleic acids. Provided as a lyophilized powder, this enzyme is ideal for large-volume applications in molecular biology and biochemistry research.

Key Features:

1. High Enzymatic Activity:
   • Activity: Approximately 20,000 units/mg dry weight, delivering reliable and consistent lysis results.
   • Efficiently hydrolyzes peptidoglycan in bacterial cell walls, targeting 1,4-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
2. Versatile Applications:
   • Facilitates the lysis of Gram-positive bacteria and improves the lysis of Gram-negative bacteria (e.g., E. coli, Salmonella, Shigella) when combined with nucleases like DNase I.
   • Suitable for inclusion body purification by digesting cellular debris and releasing inclusion bodies for downstream analysis.
3. High Purity and Solubility:
   • Supplied as a white, lyophilized powder.
   • Readily soluble at 5 mg/mL in 0.1M KH₂PO₄, producing a clear, colorless solution for ease of use.
4. Broad Compatibility:
   • Works seamlessly with Thermo Scientific Pierce Cell Lysis Reagents.
   • Compatible with downstream protein purification workflows, such as affinity purification for fusion-tagged recombinant proteins.
5. Stable and Convenient:
   • Long shelf life when stored at -20°C in a non-frost-free freezer.
   • Shipped on ice to preserve enzymatic activity during transit.

Applications:

1. Protein Extraction:
   • Breaks down bacterial cell walls to release soluble proteins for research and industrial purposes.
2. Nucleic Acid Extraction:
   • Enhances the yield of high-quality DNA and RNA from bacterial cells for genomic and transcriptomic studies.
3. Inclusion Body Purification:
   • Efficiently digests cellular debris to isolate inclusion bodies for recombinant protein studies.
4. Biochemical Research:
   • Studies enzymatic interactions with bacterial cell walls.
   • Supports structural and functional analysis of bacterial peptidoglycan.

Specifications:

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze a decrease in absorbance at 450 nm of 0.001/min at 25°C, pH 6.24, using a ~0.25 mg/mL suspension of Micrococcus luteus.

Advantages:

1. High Efficiency:
   • Quickly lyses bacterial cells, significantly improving protein and nucleic acid extraction efficiency.
2. Convenience:
   • Lyophilized powder format allows for easy preparation and storage.
   • Soluble and compatible with various buffers and workflows.
3. Broad Application Range:
   • Suitable for both research-scale and large-volume applications in molecular biology, microbiology, and biochemistry.
4. Reliability:
   • Manufactured under strict quality controls to ensure consistent performance.

Usage Instructions:

1. Dissolve the lyophilized powder in an appropriate buffer (e.g., 0.1M KH₂PO₄) at the desired concentration.
2. Add the solution to the bacterial suspension and incubate under optimal conditions for cell lysis.
3. Proceed with downstream applications, such as protein purification or nucleic acid isolation.

Related Products:

• Lysozyme Solution (50 mg/mL) for ready-to-use cell lysis.
• Thermo Scientific Pierce Cell Lysis Reagents for optimized protein extraction workflows.

The Thermo Scientific™ Lysozyme (Lyophilized Powder, 5 g) offers a reliable, high-activity solution for bacterial cell lysis and biomolecule extraction. Its compatibility with large-volume applications and downstream workflows makes it an indispensable tool for research laboratories and industrial use.