Liofilchem Ampicillin Supplement, freeze-dried

The Liofilchem® Ampicillin Supplement is a freeze-dried preparation specifically designed for use with Liofilchem® Aeromonas Agar Base (REF 610048/620048) to enhance the selective isolation of Aeromonas species from water sources (e.g., spring and bottled water) and food samples. It works by inhibiting non-target microorganisms, while supporting the growth of ampicillin-resistant Aeromonas spp.

Key Features

• Selective Enrichment: Ampicillin inhibits non-target microorganisms, facilitating the recovery of Aeromonas spp.
• Broad-Spectrum Action: Effective against a range of Gram-positive and Gram-negative bacteria, allowing for specific Aeromonas isolation.
• Pre-dosed Convenience: Supplied in freeze-dried vials for accurate dosing and reproducibility.
• Wide Applications: Ideal for environmental, water quality, and food microbiology testing.

Kit Contents

• 10 vials of freeze-dried Ampicillin Supplement
• Each vial contains 2.5 mg of ampicillin, sufficient for enriching 500 mL of Aeromonas Agar Base.

Principle of the Method

Ampicillin, a beta-lactam antibiotic, selectively suppresses Gram-positive and Gram-negative bacteria that are sensitive to its action. This allows ampicillin-resistant Aeromonas species to grow, ensuring their reliable isolation from mixed microbial populations.

Composition

Procedure for Use

1. Reconstitution:
   • Aseptically reconstitute one vial with 2 mL of sterile distilled water.
   • Shake gently until fully dissolved, avoiding foam formation.
2. Enrichment:
   • Add the reconstituted supplement (2 mL) to 500 mL of Liofilchem® Aeromonas Agar Base.
3. Mixing:
   • Stir gently to ensure uniform distribution.
4. Dispensing:
   • Pour the enriched medium into sterile Petri dishes and allow it to solidify.

Technique and Results Interpretation

• Inoculation: Follow the inoculation procedure as outlined in the technical documentation for Liofilchem® Aeromonas Agar Base (REF 610048/620048).
• Incubation: Incubate plates at 30°C for 24 hours under aerobic conditions.
• Expected Results:
  • Aeromonas hydrophila (ATCC® 7966): Good growth.
  • Pseudomonas aeruginosa (ATCC® 27853): Good growth.
  • Staphylococcus aureus (ATCC® 25923): Inhibited.

Quality Control

Prepared plates should be tested for appearance and microbial growth consistency before use.

Applications

1. Water Quality Testing:
   • Detect and isolate Aeromonas spp. in spring water, bottled water, and other environmental samples.
2. Food Microbiology:
   • Identify Aeromonas contamination in food products to ensure compliance with safety standards.
3. Environmental Monitoring:
   • Assess microbial populations in natural or industrial aquatic environments.

Storage and Stability

• Store freeze-dried vials at 2–8°C in their original packaging.
• Protect from heat, light, and temperature fluctuations.
• Do not use beyond the expiration date indicated on the label.

Precautions

• For professional use only; trained personnel must perform all procedures.
• Follow proper aseptic techniques and laboratory safety protocols.
• Refer to the Safety Data Sheet (SDS) for handling and hazard information.

Presentation and Packaging

• Product: Liofilchem® Ampicillin Supplement
• Reference: 81001
• Packaging: 10 bottles per kit, with each bottle sufficient to enrich 500 mL of medium.

References

1. Buchanan, R. L., and Palumb S. A. (1985). J. Food Saf. 7: 15–29.
2. Havelaar, A. H., During, M., Versteegh, J. F. M. (1987). J. Appl. Bact. 62: 279–287.
3. Ryan, N. (1985). In Handbook of Microbiological Media, 2nd ed.

The Liofilchem® Ampicillin Supplement is an essential tool for microbiological laboratories seeking the selective isolation of Aeromonas spp. Its high specificity, reliability, and compatibility with Liofilchem® Aeromonas Agar Base make it indispensable for environmental, water, and food testing applications.