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Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Brand | TakaRa Bio |
| Product Grade | Research Grade |
The Lenti-X p24 Rapid Titer Kit allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a standard ELISA. The wells of the included microtiter plate (12 x 8-well strips) are coated with anti-HIV-1 p24 capture antibody, which binds the p24 (an HIV-1 core protein) in your test samples. Specifically-bound p24 is detected in a typical "sandwich" ELISA format using a biotinylated anti-p24 secondary antibody, a streptavidin-HRP conjugate, and a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content.
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