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Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP, 1mL
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Specifications:
| Application | Western Blot, ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Secondary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
HRP-Conjugated Polyclonal Secondary Antibody for Western Blot, ELISA, and Colorimetric Detection
The Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP is a highly sensitive, horseradish peroxidase-conjugated (HRP) polyclonal antibody developed to recognize mouse IgG heavy and light chains. This antibody is ideal for use in Western blotting, ELISA, and other immunoassays where chemiluminescent or colorimetric detection is required.
Affinity-purified and validated for a wide range of applications, this secondary antibody delivers high signal intensity and low background, making it an excellent choice for detecting mouse primary antibodies across research workflows.
Key Features and Benefits
-
Broad Mouse IgG Reactivity
Recognizes both heavy (H) and light (L) chains of mouse IgG, including all isotypes. -
HRP-Conjugated for Enhanced Sensitivity
Enables chemiluminescent or colorimetric detection in Western blots and ELISAs. -
Optimized Dilution Range
Recommended for use at 1:2,000–1:10,000 for Western blot and 1:2,000–1:4,000 for ELISA. -
Affinity-Purified
Ensures high specificity and reduced cross-reactivity, improving assay performance and reproducibility. -
Stabilized Liquid Format
Supplied in PBS with BSA, glycerol, and Kathon™ CG preservative for long-term stability at 4°C.
Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 – 1:10,000 |
| ELISA | 1:2,000 – 1:4,000 |
Dilutions should be optimized for each experimental system.
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP |
| Catalog Number | 61-6520 |
| Host / Isotype | Rabbit / IgG |
| Reactivity | Mouse IgG (all isotypes, H+L) |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Buffer Composition | PBS, pH 7.4, with 4 mg/mL BSA, 40% glycerol, 0.19% Kathon™ CG |
| Storage Conditions | 4°C (Do not freeze) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533933 |
| Product Type | Secondary Antibody |
| Unit Size | 1 mL |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Target Information – Mouse IgG (H+L)
This secondary antibody binds specifically to mouse immunoglobulin gamma chains (IgG) and light chains (kappa and lambda). It can be used with mouse monoclonal and polyclonal primary antibodies in a variety of applications, enhancing detection sensitivity through signal amplification.
Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP is a versatile and robust reagent for the sensitive detection of mouse antibodies in Western blot and ELISA. Its broad reactivity, strong signal output, and batch-to-batch consistency make it an indispensable tool for immunodetection workflows across research disciplines.