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Specifications:
Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen™ Human BD Fc Block™. Human peripheral blood mononuclear cells were either not treated (dashed line histogram) or preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/ 564220; solid line histogram). The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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Richard Atule - bought BD Pharmingen™ Human BD Fc Block™, 50 µg