Human Anti-S. Pneumococcal Vaccine (Pcv-13) Igg Elisa Kit, 96 Tests, Quantitative

The Human Anti-S. Pneumococcal Vaccine (PCV-13) IgG ELISA Kit is a highly specific and sensitive assay designed for the quantitative detection of IgG antibodies produced in response to the 13-valent pneumococcal conjugate vaccine (PCV-13). This kit is a vital tool for assessing vaccine efficacy, monitoring immune responses, and conducting research on Streptococcus pneumoniae immunization.

Purpose

• Quantification of Anti-PCV-13 IgG Antibodies:
  • Measures IgG levels to evaluate immune responses to the PCV-13 vaccine in human samples.
• Sample Compatibility:
  • Designed for human serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to provide accurate and reproducible results. Below is the detailed mechanism:

1. Pre-Coated Plate:
   • Microtiter plate wells are pre-coated with pneumococcal antigens corresponding to PCV-13 vaccine serotypes.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing Anti-PCV-13 IgG antibodies in the sample to bind to the immobilized antigens.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated anti-human IgG antibody is added, binding to the captured IgG antibodies.
4. Washing Step:
   • Unbound components are washed away to reduce background interference.
5. Substrate Reaction:
   • TMB substrate is added, reacting with HRP to produce a blue color in positive wells.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color to yellow. The optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Concentration Determination:
   • Anti-PCV-13 IgG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of Anti-PCV-13 IgG with minimal cross-reactivity.
2. Quantitative Results:
   • Provides precise IgG measurement using a standard curve.
3. Wide Sample Compatibility:
   • Suitable for serum, plasma, and other biological fluids.
4. Reproducibility:
   • Ensures consistent results across multiple assays.
5. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.

Kit Components

• Pre-coated Microelisa strip plate with PCV-13 antigens.
• Standards for generating a standard curve.
• HRP-conjugated anti-human IgG detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Vaccine Efficacy Research:
  • Assess the effectiveness of the PCV-13 vaccine by measuring IgG antibody responses.
• Immunological Monitoring:
  • Monitor immune responses in vaccinated individuals.
• Epidemiological Studies:
  • Study population-level immune responses to pneumococcal vaccines.
• Clinical Diagnostics:
  • Support diagnostic research for immune deficiencies or suboptimal vaccine responses.
• Therapeutic Research:
  • Evaluate therapeutic interventions targeting pneumococcal infections.

Advantages

1. Accurate and Reliable:
   • High specificity ensures minimal cross-reactivity and accurate quantification.
2. Convenient:
   • Ready-to-use reagents simplify the assay protocol.
3. Versatile:
   • Compatible with various sample types and suitable for diverse research applications.
4. Reproducible:
   • Delivers consistent results for reliable data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated anti-human IgG detection antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate Anti-PCV-13 IgG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles.

The Human Anti-S. Pneumococcal Vaccine (PCV-13) IgG ELISA Kit is an essential tool for research into pneumococcal vaccine efficacy, immune responses, and public health. Its sensitivity, specificity, and user-friendly design make it an invaluable resource for clinical and epidemiological studies.