Agilent D1000 High Sensitivity DNA ScreenTape Analysis, 112 DNA Samples
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For the high sensitivity analysis of DNA from 35 to 1000 bp, includes 7 ScreenTape devices, sufficient for 112 samples.
Specifications:
Application | Capillary Electrophoresis | ||
Storage Temperature | 2-8°C | ||
Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
Product Brand | Agilent | ||
Product Grade | Molecular Biology | ||
The Agilent D1000 High Sensitivity DNA ScreenTape Analysis Kit (5067-5584) is an advanced electrophoresis solution designed for high-sensitivity analysis of DNA fragments ranging from 35 to 1000 base pairs (bp). This kit is optimized for use with Agilent TapeStation Systems, enabling automated, high-throughput DNA quality control (QC) with minimal hands-on time.
The D1000 High Sensitivity DNA ScreenTape provides accurate, reproducible analysis of low-concentration DNA samples, making it ideal for next-generation sequencing (NGS) library QC, PCR fragment analysis, and genomic DNA integrity assessment. Each kit includes 7 ScreenTape devices, sufficient to process 112 DNA samples efficiently.
Key Features & Benefits
- High-Sensitivity DNA Analysis from 35 to 1000 bp
- Detects low-concentration DNA samples with high precision.
- Ideal for quality control of NGS libraries and small DNA fragments.
- Automated Sample Processing with TapeStation Systems
- Provides standardized, reproducible DNA fragment analysis.
- Eliminates manual gel preparation and variability in results.
- Preloaded ScreenTape for Minimal Hands-On Time
- Ready-to-use disposable ScreenTape devices simplify workflows.
- Reduces sample processing time and improves lab efficiency.
- Compatible with Agilent 4150 & 4200 TapeStation Systems
- Seamlessly integrates into NGS, PCR, and genomic research workflows.
- Ensures high-quality DNA QC for sequencing and molecular biology applications.
- Cost-Effective & High-Throughput Analysis
- Processes 112 samples per kit with consistent per-sample costs.
- Enables quick analysis in as little as 1–2 minutes per sample.
Product Specifications
Feature | Details |
---|---|
Product Name | Agilent D1000 High Sensitivity DNA ScreenTape Analysis Kit |
Catalog Number | 5067-5584 |
DNA Size Range | 35 – 1000 bp |
Number of ScreenTape Devices | 7 |
Number of Samples | 112 Samples |
Compatibility | Agilent 4150 TapeStation, Agilent 4200 TapeStation |
Sensitivity | High Sensitivity for Low DNA Concentrations |
Sample Volume Required | 1–2 µL per sample |
Typical Run Time | 1–2 minutes per sample |
For Use With | DNA Fragment Analysis, NGS Library QC, PCR QC |
Storage Conditions | 4–25°C, Protect from Light |
Shipping Conditions | Ambient Temperature |
Use Cases & Applications
1. Next-Generation Sequencing (NGS) Library Quality Control
- Ensures Accurate Fragment Size Distribution
- Essential for QC of sequencing libraries before downstream applications.
- Detects adapter dimers, incomplete ligation products, and low-yield libraries.
- Validates NGS Libraries for Optimal Sequencing Performance
- Avoids unexpected sequencing failures due to poor library quality.
- Provides precise DNA integrity assessment before sequencing runs.
2. PCR Fragment Analysis & Amplicon Size Determination
- Rapid PCR Product Validation
- Confirms PCR fragment sizes without the need for manual agarose gels.
- Allows for quick troubleshooting of amplification efficiency.
- Sensitive DNA Detection in Low-Concentration Samples
- Ideal for qPCR, multiplex PCR, and forensic DNA applications.
- Differentiates specific amplicons from non-specific byproducts.
3. Genomic DNA & Plasmid Integrity Assessment
- Monitors DNA Fragmentation & Quality in Experimental Samples
- Evaluates DNA degradation levels for molecular biology workflows.
- Detects sample contamination and degradation in long-read sequencing applications.
- Used in CRISPR & Gene Editing Research
- Confirms gene knockout or insertion success in CRISPR workflows.
- Identifies unexpected DNA modifications or off-target effects.
4. Epigenetics & Low-Input DNA Research
- Ideal for Low-Input Samples & Epigenetic Studies
- Provides accurate DNA integrity data for methylation and bisulfite sequencing workflows.
- Detects low-abundance nucleic acids from clinical or rare samples.
- Facilitates Cancer Genomics & Circulating DNA Research
- Suitable for cell-free DNA (cfDNA) and circulating tumor DNA (ctDNA) analysis.
- Helps evaluate fragmented DNA samples from liquid biopsies.
Instructions for Use
Step-by-Step Protocol for TapeStation Analysis
- Load Samples & ScreenTape Device:
- Place 1–16 samples in the TapeStation sample tray.
- Insert one D1000 High Sensitivity DNA ScreenTape device.
- Start Automated Analysis:
- Launch the TapeStation software and select the D1000 High Sensitivity DNA Assay.
- The system automatically processes samples in 1–2 minutes per sample.
- Interpret & Export Data:
- View electropherogram traces & gel-like images for fragment size analysis.
- Export RINe, DIN values, or detailed fragment size data for reporting.
Storage & Handling Instructions
- Storage Conditions:
- Store at 4–25°C, protected from light.
- Do not freeze, as it may affect ScreenTape performance.
- Handling Precautions:
- Use aseptic techniques when handling DNA samples.
- Ensure ScreenTape devices remain sealed until use to prevent contamination.
- Disposal Guidelines:
- Dispose of used ScreenTape devices as per laboratory biosafety regulations.
- Follow institutional waste management protocols for electronic lab consumables.
Why Choose Agilent D1000 High Sensitivity DNA ScreenTape Analysis?
✅ Rapid, automated DNA quality assessment in under 20 minutes
✅ Eliminates manual gel preparation, reducing hands-on time
✅ Preloaded ScreenTape devices for easy, contamination-free analysis
✅ Accurate fragment size detection for NGS, PCR, and cfDNA workflows
✅ Seamless integration with Agilent TapeStation for scalable sample processing
The Agilent D1000 High Sensitivity DNA ScreenTape Analysis Kit is an essential tool for DNA quality control, enabling researchers to quickly assess sample integrity and fragment distribution with minimal hands-on time.