Invitrogen™ ERCC RNA Spike-In Mix Kit

The Invitrogen™ ERCC RNA Spike-In Mix is a standardized set of external RNA controls designed to enable accurate normalization, validation, and performance assessment in gene expression experiments. Developed by the External RNA Controls Consortium (ERCC) and traceable to NIST-certified DNA plasmids, this mix contains a defined set of synthetic RNA transcripts that mimic natural eukaryotic mRNA. These spike-in controls provide a reliable reference for evaluating RNA sequencing (RNA-Seq), qRT-PCR, and microarray workflows.

This kit includes a pre-formulated blend of 92 polyadenylated RNA transcripts, ranging from 250 to 2,000 nucleotides in length, representing a broad dynamic range of expression levels. By adding the spike-in mix to RNA samples after isolation, researchers can assess analytical performance metrics such as sensitivity, dynamic range, reproducibility, and differential gene expression accuracy across experiments and platforms.

The ERCC RNA Spike-In Mix enables standardized comparison of gene expression data between different samples, experiments, and sequencing platforms. It supports next-generation sequencing applications and microarray technologies, providing confidence in experimental results and ensuring reproducibility across laboratories.

Manufactured under strict quality standards and offered under the trusted Ambion™ product line, this spike-in mix is essential for transcriptomics, genomics, and molecular biology research requiring precise gene expression quantification and normalization.

Key Features and Benefits

• Contains 92 synthetic RNA transcripts for accurate gene expression control
• Enables normalization and comparison across RNA experiments and platforms
• Helps measure sensitivity, detection limits, and dynamic range
• Supports accurate quantification of differential gene expression
• Traceable to NIST-certified DNA reference materials
• Compatible with RNA-Seq, microarray, and qRT-PCR workflows
• Mimics natural eukaryotic mRNA with polyadenylated transcripts
• Provides standardized reference for transcriptome analysis

Applications

• RNA sequencing (RNA-Seq) normalization and validation
• Gene expression profiling and quantification
• Transcriptome analysis and differential expression studies
• Microarray and next-generation sequencing workflows
• RNA quality control and experimental validation
• Molecular biology and genomics research
• Platform performance evaluation and cross-experiment comparison

Kit Contents

Technical Specifications

Ordering Information

The Invitrogen ERCC RNA Spike-In Mix is a critical quality control tool for RNA sequencing and gene expression analysis. By providing standardized, traceable RNA controls, it enables accurate normalization, performance validation, and reproducible comparison across experiments and platforms. This kit is essential for researchers conducting transcriptomics, genomics, and molecular biology studies requiring reliable and quantitative gene expression data.