Applied Biosystems™ Region of Interest (ROI) and Background Plates, 384-well

The Applied Biosystems™ Region of Interest (ROI) and Background Plates are specialized calibration plates designed for maintaining and calibrating ViiA™ 7 System and QuantStudio™ 5, 6, 7, and 12K Flex real-time PCR systems with 384-well blocks. These plates help to ensure the accurate performance of your qPCR systems, and are primarily used for Region-of-Interest (ROI) calibration to guarantee consistent results in high-precision experiments.

The plates come pre-loaded with calibrated dyes (FAM, LIZ, NED, ROX, and VIC) that are commonly used in multiplex PCR applications. These calibration plates are essential for maintaining the integrity of PCR assays and ensuring optimal instrument performance in terms of signal detection and fluorescence accuracy.

Key Features & Benefits

✅ Calibrated for ROI and Background Calibration

• Specifically designed to support Region-of-Interest (ROI) calibration, which is crucial for accurate fluorescence detection in qPCR assays.
• Used to maintain optimal performance of real-time PCR systems (ViiA™ 7 and QuantStudio™ series).

✅ Multiple Calibrated Dyes

• Plates come pre-loaded with FAM, LIZ, NED, ROX, and VIC dyes, which are standard dyes used for fluorescence-based detection in multiplex qPCR assays.
• These dyes allow for precise calibration of the system across multiple channels, ensuring accurate detection and quantification of nucleic acids.

✅ 384-Well Plate Format

• Designed for use with 384-well blocks, offering high-throughput capabilities for multiplex assays, high-resolution data, and efficient sample analysis.
• Ideal for high-volume qPCR applications, where fast, reproducible results are needed.

✅ Easy Instrument Maintenance

• The ROI and Background Plates are used for routine system maintenance, ensuring that the instrument's optical system is operating within specifications for accurate data collection.
• Simple to use—no complex setup or reagents required.

✅ Storage & Shipping

• Plates should be stored at –15°C to –25°C for long-term storage and stability.
• Shipped with dry ice to preserve the integrity of the calibration dyes and prevent degradation.

Specifications

Applications

• Real-Time PCR Calibration:
  • Ensures precise fluorescence detection in multiplex PCR assays and qPCR experiments.
• Instrument Maintenance:
  • Used for routine calibration of ViiA™ 7 and QuantStudio™ systems, ensuring reliable performance during high-throughput qPCR experiments.
• Multiplex PCR Assays:
  • Ideal for labs performing multiplex gene expression studies or genetic variation detection using multiple fluorescent dyes.
• Routine Quality Control:
  • Essential for labs requiring consistent performance in real-time PCR assays, especially those performing quantitative and qualitative analyses.

Why Choose Applied Biosystems™ ROI and Background Plates?

✅ Ensure accuracy and reliability in multiplex PCR assays with multiple dye calibration.

✅ Easy and fast maintenance of ViiA™ 7 and QuantStudio™ PCR systems.

✅ 384-well format for high-throughput data collection.

✅ Routine calibration for consistent, reproducible qPCR results.

✅ Stable storage conditions and dry ice shipping to maintain dye integrity.