VWR Folin-Denis' reagent for analysis of uric acid

For the Colorimetric Analysis of Uric Acid and Polyphenols

VWR Folin-Denis' Reagent is a ready-to-use colorimetric reagent primarily used in biochemical and clinical assays for the determination of uric acid and polyphenolic compounds. This reagent is a classical analytical solution containing phosphomolybdotungstic acid, which forms a blue complex when reduced by phenolic substances or uric acid under alkaline conditions. The intensity of the resulting blue color is measured spectrophotometrically and is directly proportional to the concentration of the analyte.

Key Features

• Pre-formulated for convenience: Saves preparation time and reduces handling of hazardous chemicals
• Sensitive and reliable: Suitable for detecting microgram levels of uric acid or phenolic content
• Colorimetric reaction: Produces a quantifiable blue complex under alkaline conditions
• Wide applicability: Used in clinical diagnostics, nutritional studies, and plant metabolite analysis

Applications

• Quantification of uric acid in blood and urine (clinical chemistry)
• Measurement of total polyphenols in plant extracts, foods, and beverages
• Antioxidant studies involving phenolic content evaluation
• Academic and industrial research in biochemistry and food science

Storage and Handling

• Store in a cool, dark place away from heat and direct sunlight
• Use appropriate PPE when handling (acidic and oxidative components present)
• Dispose of according to local chemical waste regulations

Compatible Methods

• Folin-Denis Method for total phenol content
• Colorimetric uric acid assay (reaction under alkaline conditions with sodium carbonate or sodium hydroxide)
• Detection via spectrophotometer at λ ~ 700 nm