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Thermo Scientific™ n-octyl-β-D-thioglucopyranoside (OTG) ≥ 99%, 5g
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Specifications:
| Application | Protein Biology, Protein crystallography | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₁₄H₂₈O₅S |
Enzyme-Stable, Non-Ionic Detergent for Protein Solubilization and Membrane Biology Applications
Thermo Scientific™ Octylthio-β-D-glucoside (OTG) is a high-purity, non-ionic detergent specifically designed for gentle protein solubilization in biochemical and structural biology workflows. As a thioglucoside analog of octyl-β-D-glucoside, OTG features a sulfur atom in place of the glycosidic oxygen bond, providing enhanced resistance to β-D-glucosidase hydrolysis and extending its stability in enzymatically active environments.
OTG is ideal for applications requiring mild, non-denaturing conditions, such as membrane protein extraction, liposome work, and enzyme stabilization, especially where conventional glucosides may degrade. Its low critical micelle concentration (CMC), high solubility, and optical clarity at 280 nm make it suitable for spectroscopy, chromatography, and structural studies.
Key Features & Benefits
- Enzyme-Resistant Structure – Thioether linkage protects against enzymatic hydrolysis by β-D-glucosidases
- Non-Ionic & Mild – Maintains protein structure and function during solubilization
- Low CMC (9 mM) – Effective micelle formation at low concentrations
- High Purity & Optical Clarity – Minimal UV absorbance for sensitive detection methods
- Versatile Use – Ideal for applications in proteomics, lipid membrane research, and enzyme assays
Applications
- Membrane Protein Extraction – Solubilizes transmembrane proteins under non-denaturing conditions
- Enzyme-Active Systems – Suitable for workflows where traditional glucosides degrade
- Liposome Preparation – Useful in forming stable lipid-based vesicles
- Spectrophotometric Assays – Compatible with UV-based detection due to low absorbance
- Detergent-Sensitive Protein Studies – Enables solubilization without interfering with activity
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Octylthio-β-D-glucoside (OTG) |
| Catalog Number | 28351 |
| Chemical Name | n-Octyl-β-D-thioglucopyranoside |
| Molecular Formula | C₁₄H₂₈O₅S |
| Molecular Weight | ~444 g/mol |
| Form | Lyophilized powder |
| Detergent Class | Non-ionic |
| Critical Micelle Concentration (CMC) | ~9 mM (0.2772% w/v) |
| Cloud Point | >100°C |
| UV Absorbance (280 nm) | Very low |
| Storage Conditions | Store at 2-8°C in a dry, light-protected area |
| Solubility | Readily soluble in water |
| Purity | High-purity, research-grade |
| Regulatory Use | For Research Use Only |
| Quantity | 5 g |
Handling & Storage
- Store at 2-8°C, protected from moisture and light
- Dissolve in water or compatible buffer just before use
- Avoid repeated freeze-thaw cycles
- Use appropriate PPE when handling lyophilized powders
Thermo Scientific™ Octylthio-β-D-glucoside (OTG) offers a robust alternative to traditional glucosides for researchers working with delicate membrane proteins, enzymes, or lipid systems. Its resistance to enzymatic degradation, combined with its non-denaturing properties and high solubility, makes it an indispensable tool for membrane biology, protein purification, and structural research under sensitive conditions.