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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Enzymes and Substrates | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-contrast chromogenic substrate for β-galactosidase detection
Invitrogen™ Bluo-Gal is a halogenated indolyl-β-galactoside chromogenic substrate used for the detection of β-galactosidase activity in blue/white screening, viral plaque assays, and histochemical staining. Upon hydrolysis by β-galactosidase, Bluo-Gal produces an intense dark blue precipitate that is more saturated in color than X-Gal, improving visual contrast in screening and detection workflows.
Bluo-Gal can also be used with streptavidin–β-galactosidase conjugates in immunoblotting and immunocytochemistry assays. The insoluble blue reaction product is stable and resistant to alcohols and xylenes, ensuring compatibility with a variety of fixation and staining protocols.
Key Features
- Darker Blue Signal – Produces more intense coloration than X-Gal
- Versatile Applications – Suitable for blue/white screening, histochemistry, and overlay assays
- Stable Precipitate – Insoluble in alcohols and xylenes for robust staining
- Compatible with β-Gal Reporter Systems – Works in both prokaryotic and eukaryotic expression systems
- Soluble in DMF – Easy preparation of concentrated stock solutions
Applications
- Blue/white colony screening to identify recombinant vs. non-recombinant colonies
- Viral plaque assays to detect lac+ viral plaques
- Histochemical β-galactosidase staining in fixed tissues
- Immunoblotting and immunocytochemistry with β-galactosidase conjugates
- Bluo-Gal overlay assays for β-gal activity detection
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | 15519028 |
| Chemical Name | Halogenated Indolyl-β-galactoside |
| Quantity | 1 g |
| Detection Method | Colorimetric |
| Format | Powder, bottle |
| Target Enzyme | β-galactosidase |
| Shipping Conditions | Room temperature or wet ice |
| Storage Conditions | –5°C to –30°C |
| Regulatory Status | Research Use Only (RUO) |
Bluo-Gal is an excellent alternative to X-Gal for β-galactosidase detection, providing richer color development and enhanced contrast for reliable visual identification in colony screening, histological staining, and immunoassay applications.