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Thermo Scientific™ Gibco™ LHC-9 Basal Medium (1X)
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With Glutamine and Phenol Red
storage conditions: -5°C to -20°C.
Specifications:
| Application | Cell culture | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ LHC-9 Medium (1X) is a ready-to-use, serum-free culture medium specifically formulated for the growth and maintenance of bronchial epithelial cells, including immortalized and primary cell lines such as IB3-1, BEAS-2B, NHBE, BZR, BBM, Het-1A, and C38. This medium supports research in asthma, allergy, cystic fibrosis, lung and esophageal cancers, and other pulmonary-related studies.
Developed to deliver high reproducibility without the need for additional supplements, LHC-9 Medium provides a convenient, consistent, and defined environment for culturing epithelial cells. It is performance-tested using BEAS-2B cells and includes Glutamine and Phenol Red to support cellular growth and pH monitoring.
Key Features
- Serum-free formulation – reduces variability and risk of contamination
- Ready-to-use 1X liquid medium – no need for reconstitution or further supplementation
- Optimized for bronchial epithelial cells including both human (NHBE) and bovine (BBE) sources
- Supports a wide range of pulmonary research (e.g., asthma, cancer, fibrosis)
- Contains glutamine and phenol red
- Sterile-filtered to ensure cell culture compatibility
- Validated using BEAS-2B cell growth assays for consistent performance
Applications
- Culture of bronchial epithelial cells (e.g., NHBE, BEAS-2B, IB3-1, BZR, C38)
- Research in asthma, allergy, cystic fibrosis, and respiratory cancers
- Ideal for use in toxicology, drug testing, and respiratory disease models
- Suitable for both primary and immortalized epithelial cell lines
Specifications
| Property | Details |
|---|---|
| Product Name | Gibco™ LHC-9 Medium (1X) |
| Catalog Number | 12680013 |
| Format | Liquid, ready-to-use |
| Concentration | 1X |
| Classification | Serum-free |
| Additives Included | Glutamine, Phenol Red |
| Cell Types Supported | Bronchial epithelial cells |
| Compatible Cell Lines | IB3-1, S9, C38, BEAS-2B, NHBE, BZR, BBM, Het-1A, BBE |
| Culture Type | Mammalian epithelial cells |
| Volume | 500 mL |
| Sterility | Sterile-filtered |
| Shelf Life | 12 months |
| Storage Conditions | –5 to –30 °C; protect from light |
| Shipping Conditions | Dry ice |
| Research Use | For Research Use Only. Not for use in diagnostic procedures. |
Reference Publications
- Lechner & LaVeck, J Tissue Cult Methods (1985)
- Zhao et al., Carcinogenesis (2000)
- Hei et al., Environ Health Perspect (1997)
- Pawliczak et al., J Biol Chem (2001)
Gibco™ LHC-9 Medium (1X) provides a serum-free, high-performance solution for culturing bronchial epithelial cells in advanced respiratory and cancer research. With consistent results, pre-added supplements, and long shelf life at –20°C, LHC-9 enables researchers to maintain healthy, reproducible cultures with reduced variability and time savings.