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Gibco™ Cholesterol Lipid Concentrate (250X), 100mL
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Cholesterol Lipid Concentrate (250X) is a chemically defined, protein-free supplement designed to support the growth and productivity of cholesterol-dependent cell lines, including NS0, CHO, and other mammalian or insect cell cultures. This sterile, ready-to-use solution enhances cell viability and protein expression in serum-free and chemically defined media—without introducing complex components that interfere with downstream purification.
Formulated to be easily added to basal media such as Gibco™ CD Hybridoma Medium, this concentrate supports high-density growth, particularly for NS0 hybridoma cells, and maintains lipid bioavailability through filtration with minimal lipid loss.
Key Features
- 250X Concentration: Highly concentrated formulation minimizes volume needed in culture systems.
- Chemically Defined & Protein-Free: Ensures consistency and eliminates animal-derived variability.
- Enhances Lipid-Dependent Growth: Ideal for NS0 and similar cholesterol-requiring lines.
- Filtration-Friendly: Can be sterile-filtered with negligible lipid loss, ensuring aseptic workflows.
- Compatible with Serum-Free Media: Enhances productivity in chemically defined media platforms.
Applications
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Support of Cholesterol-Dependent Cell Lines:
- NS0 and other hybridomas requiring exogenous cholesterol.
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Recombinant Protein Production:
- Enables high-yield expression in serum-free culture systems.
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Custom Media Optimization:
- Can be integrated into feeding strategies or customized formulations via Gibco™ Custom Products Services.
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Hybridoma and Myeloma Antibody Production:
- Enhances growth in chemically defined antibody production systems.
Specifications
| Attribute | Details |
|---|---|
| Product Name | Cholesterol Lipid Concentrate (250X) |
| Catalog Number | 12531018 |
| Cell Types | Mammalian, Insect |
| Culture Type | Mammalian Cell Culture, Insect Cell Culture |
| Concentration | 250X |
| Form | Liquid |
| Sterility | Sterile |
| pH Range | 6.0 to 8.0 |
| Additives | Cholesterol and defined lipids (protein-free) |
| Storage Conditions | 2–8°C; protect from light |
| Shipping Conditions | Room Temperature or Wet Ice |
| Shelf Life | 18 months |
| Intended Use | Research Use Only – Not for diagnostic procedures |
Gibco™ Cholesterol Lipid Concentrate (250X) is an essential supplement for cholesterol-dependent cell lines cultured under serum-free and chemically defined conditions. Its high concentration, filtration compatibility, and protein-free formulation make it an ideal choice for maintaining robust growth and antibody production while minimizing complications in downstream purification.