Invitrogen™ eBioscience™ CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), PE, 100 Tests

The Invitrogen™ CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), conjugated to PE (Phycoerythrin), is a premium antibody designed for the detection of CD49d, also known as Integrin alpha 4. CD49d is a 150 kDa integrin alpha subunit that non-covalently associates with integrin beta1 (CD29) or beta7, forming the heterodimers VLA-4 (alpha4beta1) and alpha4beta7, which are crucial for cellular adhesion and immune response regulation. This antibody is validated for flow cytometry and provides a bright, stable fluorescence signal for accurate and reproducible data.

Key Features:

1. Target and Specificity:
   • Recognizes CD49d (Integrin alpha 4), which is involved in cellular adhesion via interaction with fibronectin, VCAM-1 (CD106), and MadCAM-1.
   • CD49d is expressed on thymocytes, peripheral lymphocytes, monocytes, and eosinophils.
2. Clone and Conjugate:
   • Clone: 9F10, ensuring high specificity and reproducibility.
   • Conjugate: PE (Phycoerythrin), offering bright fluorescence with excitation at 565 nm and emission at 578 nm.
3. Validated Applications:
   • Tested and optimized for flow cytometry, providing robust detection in human samples.
4. Pre-Titrated and Ready-to-Use:
   • Supplied as a liquid formulation, pre-titrated for convenience, with each test requiring 5 µL (0.125 µg).
5. Bright Fluorescence:
   • PE provides a high-intensity signal for clear and accurate flow cytometric analysis.

Specifications:

Applications:

1. Flow Cytometry:
   • Detects CD49d (Integrin alpha 4) expression on thymocytes, lymphocytes, monocytes, and eosinophils.
   • Useful for studying cellular adhesion and immune responses.
2. Immunophenotyping:
   • Ideal for analyzing integrin alpha 4 expression in various immune cell subsets.
3. Adhesion Molecule Research:
   • Investigate the role of CD49d in cellular adhesion and migration, particularly its interaction with fibronectin, VCAM-1, and MadCAM-1.
4. Cancer and HIV Research:
   • Study the role of integrin alpha4beta7 in cancer metastasis and its interaction with the HIV-1 envelope.
5. Stem Cell and Tissue Studies:
   • Examine CD49d-mediated mesenchymal stem cell aggregation and its role in tissue adhesion.

Key Benefits:

1. High Specificity and Sensitivity:
   • The 9F10 clone provides reliable detection of CD49d with minimal background noise.
2. Bright and Stable Signal:
   • PE conjugate ensures high fluorescence intensity for clear separation of positive and negative populations.
3. Reproducible Results:
   • Validated for flow cytometry, guaranteeing consistent and reliable data.
4. Ease of Use:
   • Pre-titrated and ready-to-use formulation simplifies experimental workflows.
5. Versatile Research Tool:
   • Suitable for studies in immunology, cancer, adhesion biology, and infectious diseases.

Usage Recommendations:

1. Sample Preparation:
   • Stain 10⁵–10⁸ cells per test in a final volume of 100 µL using 5 µL of antibody solution.
2. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a blue laser (488 nm), green laser (561 nm), or yellow-green laser.
3. Fixation:
   • Use appropriate fixation buffers for optimal fluorescence preservation.

Storage and Handling:

• Store at 4°C in the dark to protect the PE fluorochrome.
• Do not freeze, as this may compromise the performance of the antibody.

Target Information:

• CD49d (Integrin alpha 4):
  • A member of the integrin family that associates with beta1 or beta7 to form heterodimers.
  • Plays a crucial role in cellular adhesion, migration, and immune cell trafficking.
  • Interacts with fibronectin, VCAM-1, and MadCAM-1, facilitating adhesion to vascular endothelium and mucosal tissues.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.