Thermo Scientific™ Sodium Pyruvate (100 mM), Sterile-filtered

The Thermo Scientific™ Sodium Pyruvate (100 mM) is a sterile, ready-to-use solution designed to supplement cell culture media. Sodium pyruvate serves as an alternative carbon source, enhancing cellular energy metabolism and supporting optimal cell growth and viability in mammalian cell cultures.

Key Features:

1. Ready-to-Use Format:
   • Pre-prepared 100 mM solution eliminates the need for weighing and dissolving powder, saving time and ensuring accuracy.
2. Sterile and Stable:
   • Sterile-filtered to prevent contamination.
   • Shelf life of 12 months when stored at 2–8°C and protected from light.
3. Broad pH Range:
   • pH range of 6.0 to 8.0 ensures compatibility with various cell culture media formulations.
4. Ambient Shipping:
   • Shipped at ambient temperature and stored at refrigeration temperature (2–8°C) for convenience.
5. Multipurpose:
   • Suitable for a wide variety of mammalian cell types, supporting energy production and metabolic functions.

Applications:

1. Cell Culture Supplementation:
   • Provides an additional carbon source for energy production in mammalian cell cultures.
   • Enhances growth and viability of cells in media formulations lacking glucose or with limited energy substrates.
2. Metabolic Studies:
   • Investigates metabolic pathways involving glycolysis, gluconeogenesis, and the tricarboxylic acid (TCA) cycle.
3. Biomanufacturing:
   • Supports cell lines used in the production of biologics, such as monoclonal antibodies and recombinant proteins.
4. Research Applications:
   • Facilitates experiments requiring sustained energy metabolism, such as drug testing, toxicology, and physiological studies.

Specifications:

Use Cases:

1. Energy Source in Cell Culture:
   • Enhances cell survival and proliferation under low-glucose conditions by acting as a supplemental energy source in the TCA cycle.
2. Tissue Culture Maintenance:
   • Supports maintenance and long-term culturing of mammalian cells, particularly energy-demanding cell types like neurons or hepatocytes.
3. Biological Research:
   • Enables studies involving metabolic reprogramming, mitochondrial function, and oxidative phosphorylation.
4. Stress Response Studies:
   • Investigates cellular responses to metabolic stressors, such as glucose deprivation or hypoxia.
5. Protein Production:
   • Used in CHO, HEK293, or other cell lines during the production of therapeutic proteins and antibodies.

Advantages:

1. High Quality:
   • Sterile, pre-made solution ensures consistency and reduces variability in experiments.
2. Convenience:
   • Ready-to-use format eliminates the need for time-consuming preparation steps.
3. Versatile:
   • Compatible with a wide range of mammalian cell types and experimental protocols.
4. Stable and Reliable:
   • Long shelf life ensures product availability for extended use in laboratories.

Storage and Handling:

• Store at 2–8°C and protect from light to maintain stability and sterility.
• Avoid repeated freeze-thaw cycles to preserve product quality.

Product Details:

The Thermo Scientific™ Sodium Pyruvate (100 mM) is a high-quality supplement essential for maintaining cell energy metabolism and viability. Its ready-to-use format and broad applicability make it an indispensable tool for cell culture, research, and biomanufacturing applications.