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Sigma-Aldrich Ethidium bromide, (1% solution in water) for electrophoresis, 30 mL
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Specifications:
| Application | Gel Electrophoresis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Gel Stain | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | Formula | C₂₁H₂₀BrN₃ |
Sigma-Aldrich Ethidium Bromide is a ready-to-use, 1% aqueous solution optimized for nucleic acid staining in electrophoresis workflows. This high-purity fluorescent dye is widely used for visualizing DNA and RNA in agarose and polyacrylamide gels under UV light, and for quantification applications in molecular biology.
This solution offers robust performance and ease of use, making it a staple in many molecular biology protocols, particularly for DNA gel electrophoresis and small-scale transcription analysis.
Biochemical and Physiological Actions
Ethidium bromide intercalates between the base pairs of double-stranded DNA, exhibiting strong red fluorescence under UV light (emission ~605 nm, excitation ~480 nm). It is membrane-impermeable, making it effective for identifying compromised cells. As a known mutagen and carcinogen, proper laboratory handling and disposal procedures are required.
Features and Benefits
- Convenient Ready-to-Use Format: Supplied as a 1% aqueous solution for immediate use
- Reliable Visualization: High affinity for nucleic acids ensures sharp, consistent fluorescence
- Broad Compatibility: Suitable for agarose and polyacrylamide gels, as well as DNA quantification
- Validated for Electrophoresis: Passes suitability test for electrophoretic applications
Specifications
| Property | Specification |
|---|---|
| Concentration | 1% (w/v) in water |
| Form | Liquid |
| Color | Orange-red solution |
| Absorption Maximum (H₂O) | 478–482 nm |
| Specific Absorptivity (A₁%/1 cm) | 140–160 |
| UV/VIS Spectrum Identity | Passes test |
| Suitability for Electrophoresis | Passes test |
| Storage Temperature | Ambient |
| CAS Number | 1239-45-8 |
| Hazard Classification | Carcinogenic, toxic – handle with care |
Applications
- DNA visualization in agarose and polyacrylamide gel electrophoresis
- RNA and DNA detection post-electrophoresis
- DNA quantification via fluorometric techniques
- Staining of gels in transcription and PCR workflows
Sigma-Aldrich’s 1% Ethidium Bromide solution delivers consistent and reliable results for routine DNA visualization. Its high specificity, ease of use, and validated electrophoresis compatibility make it an indispensable tool for molecular biology laboratories. Strict adherence to safety guidelines is essential due to its mutagenic and carcinogenic properties.