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Varioskan™ LUX Multimode Microplate Reader (Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen with Single Dispenser)
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Reading Options: Top and Bottom
No. of dispensers: 1
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATD1) represents the pinnacle of multimode microplate detection technology, integrating five key measurement technologies — Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen™/AlphaLISA™ — in one modular, high-performance platform.
This configuration, equipped with a single precision dispenser, supports simultaneous reagent addition and signal measurement, making it ideal for kinetic assays, luminescence reactions, and AlphaScreen-based interaction studies.
Combining a xenon flash lamp and LED light source, advanced dual monochromator optics, and intelligent automation, the Varioskan LUX delivers exceptional sensitivity, reproducibility, and flexibility across a broad spectrum of life science and biochemical applications.
Key Features and Benefits
1. Five Measurement Technologies in One System
- Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF/TR-FRET), and AlphaScreen™/AlphaLISA™ detection modes.
- Top and bottom optics for enhanced assay flexibility.
- Dual monochromator system allows customizable excitation and emission wavelengths.
- Built-in AlphaScreen™ excitation LED (680 nm) for homogeneous proximity assays.
- Spectral scanning for wavelength optimization and assay development.
2. Precision Dispensing System
- One built-in dispenser (2–5,000 μL with 1 μL increments) for accurate reagent delivery.
- Enables simultaneous dispensing and signal monitoring at the start of a reaction.
- Ideal for enzyme kinetics, luminescence reactions, and AlphaScreen assays.
3. High Sensitivity and Dynamic Range
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Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well
- Bottom: <4 fmol fluorescein/well
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white low-volume 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
- AlphaScreen Sensitivity: <100 amol phosphotyrosine/well (384-well plate).
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Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- TRF: >6 decades
- Luminescence: >7 decades
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
4. Smart Automation and Reliability
- Automatic dynamic range selection ensures optimal signal detection.
- Smart Safety Controls prevent common operational errors.
- Self-calibration and diagnostics maintain long-term accuracy.
- Multiple read modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature regulation (ambient +4°C to 45°C) ensure uniform assay conditions.
5. Powerful SkanIt™ Software Integration
- Includes SkanIt™ PC Software, Research Edition for complete assay setup, control, and analysis.
- License-free installation for multi-user environments.
- Virtual Pipetting Tool simplifies plate layout and workflow design.
- Real-time visualization of spectral and kinetic curves during measurement.
- Automatic data saving ensures no data loss during interruptions.
- Built-in analytical tools: Curve fitting, standard curves, kinetic analysis, blank subtraction, and dose-response.
- Flexible export options: *.xlsx, *.pdf, *.xml, *.txt, or automatic email reports post-run.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays for TR-FRET/hTRF assays.
- DLReady™ validated by Promega Corporation for luminescence detection.
- Modular design supports upgrades and integration with automation systems.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Dispensers | 1 (2–5,000 μL, 1 μL increments) |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF Excitation: 334 nm; Emission: 400–700 nm; Luminescence: 360–670 nm; AlphaScreen Excitation: 680 nm; Emission: 400–660 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI: >6 decades (top), >5.5 decades (bottom); TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI: <0.4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well; AlphaScreen: <100 amol phosphotyrosine/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp + LED (AlphaScreen excitation) |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 58 kg (128 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- AlphaScreen™ and AlphaLISA™ Assays: Protein-protein, DNA-protein, and receptor-ligand interaction analysis.
- Fluorescence and TRF Studies: FRET/TR-FRET, HTRF®, and time-resolved signal measurement.
- Enzyme Kinetics: Real-time enzyme activity and inhibition assays with dispensing.
- Luminescent Assays: ATP quantification, luciferase, and bioluminescence assays.
- Absorbance Assays: Nucleic acid and protein quantification using UV-Vis spectroscopy.
- Spectral Scanning: Excitation/emission optimization for assay development.
- Drug Discovery: Quantitative screening for compound efficacy and target binding.
Unique Advantages
- Combines five detection technologies in one modular system.
- Integrated AlphaScreen™ LED for homogeneous proximity assays.
- Single high-precision dispenser allows reagent addition during real-time measurement.
- Dual top and bottom optics enhance detection across multiple assay types.
- HTRF™ and DLReady™ certified for validated multimode performance.
- Automatic calibration and dynamic range selection ensure high reproducibility.
- Supplied with Nunc™ Optical-Bottom Microplates for superior optical clarity and cell compatibility.
- SkanIt™ Software provides advanced data control, real-time analysis, and traceable reporting.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATD1) offers five-in-one multimode detection, integrating Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, and AlphaScreen™ in a single high-performance system.
Featuring dual optics, a built-in dispenser, and automated calibration, it delivers accurate, sensitive, and high-throughput assay performance for demanding applications in drug discovery, molecular biology, and biochemical analysis.
Paired with SkanIt™ Software (Research Edition) and Nunc™ Optical-Bottom Microplates, the Varioskan LUX ensures consistent, high-quality results, providing the complete solution for multimode microplate-based research workflows.