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The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD1) delivers unmatched flexibility, precision, and automation for multimode microplate-based assays, integrating Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF) technologies.
Equipped with a single integrated dispenser for accurate reagent addition, this configuration is ideal for enzyme kinetics, luminescent reactions, and time-critical fluorescence assays. Its dual top and bottom reading optics enable maximum assay adaptability for both homogeneous and adherent cell-based applications.
Validated for HTRF™ (Cisbio) and DLReady™ (Promega) assays, the Varioskan LUX combines advanced automation, dynamic range optimization, and SkanIt™ Software integration to simplify workflows, ensure reproducibility, and support high-throughput research environments.
Key Features and Benefits
1. Advanced Multimode Detection
- Combines Absorbance (UV-Vis 200–1000 nm), Fluorescence Intensity (incl. FRET), Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET) detection technologies.
- Top and bottom optics enhance flexibility for a wide range of assay types.
- Dual monochromator design allows precise wavelength control for excitation and emission.
- Spectral scanning supports full-wavelength analysis and assay optimization.
2. Precision Dispensing System
- Single high-precision dispenser (2–5,000 μL with 1 μL increments) enables simultaneous reagent addition and signal detection.
- Ideal for real-time kinetic monitoring in enzymatic and luminescent assays.
- Reduces manual pipetting errors and ensures consistent reaction initiation.
3. High Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well
- Bottom: <4 fmol fluorescein/well
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
-
Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- TRF: >6 decades
- Luminescence: >7 decades
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
- Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
4. Intelligent Automation and Workflow Control
- Automatic dynamic range selection optimizes well-by-well sensitivity.
- Smart Safety Controls identify setup and operational errors in advance.
- Automated calibration and diagnostics ensure accurate and stable performance.
- Multiple measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature control (ambient +4°C to 45°C) maintain consistent assay conditions.
5. SkanIt™ Software Integration
- SkanIt™ Research Edition Software included for comprehensive data management.
- License-free, multi-user installation for shared lab environments.
- Virtual Pipetting Tool enables easy experimental setup and sample mapping.
- Real-time kinetic and spectral curve display.
- Automatic data saving prevents information loss from interruptions.
- Built-in analytical functions for blank subtraction, curve fitting, standard curve, dose-response, kinetic, and spectral analyses.
- Flexible data export: *.xlsx, *.pdf, *.xml, *.txt, with optional automatic email reporting.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Upgradeable modular design for additional detection technologies such as AlphaScreen™ and AlphaLISA™.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Dispensers | 1 (2–5,000 μL, 1 μL increments) |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF: Excitation 334 nm, Emission 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 58 kg (128 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Kinetic Enzyme Assays: Real-time reaction and inhibition studies with automated dispensing.
- Cell-Based Assays: Reporter gene, cell viability, cytotoxicity, and proliferation assays.
- Time-Resolved Fluorescence (TRF): TR-FRET/hTRF and HTRF® applications for energy transfer studies.
- Luminescence Assays: ATP quantification, luciferase activity, and cell signaling.
- Spectral Scanning and Optimization: Excitation/emission profiling for assay development.
- Drug Discovery: Quantitative fluorescence and luminescence-based screening assays.
Unique Advantages
- Combines Absorbance, Fluorescence, Luminescence, and TRF in one system.
- Single integrated dispenser allows precise, automated reagent addition.
- Dual top and bottom optics maximize assay flexibility.
- HTRF™ and DLReady™ certifications ensure validated assay compatibility.
- Automatic calibration and dynamic range optimization improve reproducibility.
- Modular, upgradable design for expanding research capabilities.
- SkanIt™ Software offers real-time visualization, traceable reports, and robust data analysis.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD1) provides high-performance, four-mode detection with an integrated single dispenser for precise reagent handling.
Engineered for automation, sensitivity, and reliability, it supports a wide range of research applications including enzyme kinetics, luminescence, and TR-FRET assays.
Paired with SkanIt™ Software (Research Edition) and accompanied by Nunc™ Optical-Bottom Microplates, the Varioskan LUX ensures accurate, reproducible, and high-throughput analysis—a complete solution for modern molecular and cellular biology laboratories.
Varioskan LUX Multimode Microplate Reader Brochure.pdf
microplate instruments assays accessories.pdf
microplate readers.pdf
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD1) delivers unmatched flexibility, precision, and automation for multimode microplate-based assays, integrating Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF) technologies.
Equipped with a single integrated dispenser for accurate reagent addition, this configuration is ideal for enzyme kinetics, luminescent reactions, and time-critical fluorescence assays. Its dual top and bottom reading optics enable maximum assay adaptability for both homogeneous and adherent cell-based applications.
Validated for HTRF™ (Cisbio) and DLReady™ (Promega) assays, the Varioskan LUX combines advanced automation, dynamic range optimization, and SkanIt™ Software integration to simplify workflows, ensure reproducibility, and support high-throughput research environments.
Key Features and Benefits
1. Advanced Multimode Detection
- Combines Absorbance (UV-Vis 200–1000 nm), Fluorescence Intensity (incl. FRET), Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET) detection technologies.
- Top and bottom optics enhance flexibility for a wide range of assay types.
- Dual monochromator design allows precise wavelength control for excitation and emission.
- Spectral scanning supports full-wavelength analysis and assay optimization.
2. Precision Dispensing System
- Single high-precision dispenser (2–5,000 μL with 1 μL increments) enables simultaneous reagent addition and signal detection.
- Ideal for real-time kinetic monitoring in enzymatic and luminescent assays.
- Reduces manual pipetting errors and ensures consistent reaction initiation.
3. High Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well
- Bottom: <4 fmol fluorescein/well
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
-
Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- TRF: >6 decades
- Luminescence: >7 decades
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
- Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
4. Intelligent Automation and Workflow Control
- Automatic dynamic range selection optimizes well-by-well sensitivity.
- Smart Safety Controls identify setup and operational errors in advance.
- Automated calibration and diagnostics ensure accurate and stable performance.
- Multiple measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature control (ambient +4°C to 45°C) maintain consistent assay conditions.
5. SkanIt™ Software Integration
- SkanIt™ Research Edition Software included for comprehensive data management.
- License-free, multi-user installation for shared lab environments.
- Virtual Pipetting Tool enables easy experimental setup and sample mapping.
- Real-time kinetic and spectral curve display.
- Automatic data saving prevents information loss from interruptions.
- Built-in analytical functions for blank subtraction, curve fitting, standard curve, dose-response, kinetic, and spectral analyses.
- Flexible data export: *.xlsx, *.pdf, *.xml, *.txt, with optional automatic email reporting.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Upgradeable modular design for additional detection technologies such as AlphaScreen™ and AlphaLISA™.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Dispensers | 1 (2–5,000 μL, 1 μL increments) |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF: Excitation 334 nm, Emission 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 58 kg (128 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Kinetic Enzyme Assays: Real-time reaction and inhibition studies with automated dispensing.
- Cell-Based Assays: Reporter gene, cell viability, cytotoxicity, and proliferation assays.
- Time-Resolved Fluorescence (TRF): TR-FRET/hTRF and HTRF® applications for energy transfer studies.
- Luminescence Assays: ATP quantification, luciferase activity, and cell signaling.
- Spectral Scanning and Optimization: Excitation/emission profiling for assay development.
- Drug Discovery: Quantitative fluorescence and luminescence-based screening assays.
Unique Advantages
- Combines Absorbance, Fluorescence, Luminescence, and TRF in one system.
- Single integrated dispenser allows precise, automated reagent addition.
- Dual top and bottom optics maximize assay flexibility.
- HTRF™ and DLReady™ certifications ensure validated assay compatibility.
- Automatic calibration and dynamic range optimization improve reproducibility.
- Modular, upgradable design for expanding research capabilities.
- SkanIt™ Software offers real-time visualization, traceable reports, and robust data analysis.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD1) provides high-performance, four-mode detection with an integrated single dispenser for precise reagent handling.
Engineered for automation, sensitivity, and reliability, it supports a wide range of research applications including enzyme kinetics, luminescence, and TR-FRET assays.
Paired with SkanIt™ Software (Research Edition) and accompanied by Nunc™ Optical-Bottom Microplates, the Varioskan LUX ensures accurate, reproducible, and high-throughput analysis—a complete solution for modern molecular and cellular biology laboratories.
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