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The QuantStudio™ Absolute Q™ Digital PCR (dPCR) System revolutionizes digital PCR by combining the absolute quantification capabilities of dPCR with the simplicity and speed of a qPCR workflow. Designed for researchers who need precise, sensitive, and reproducible quantification, the Absolute Q system integrates all dPCR steps—compartmentalization, thermal cycling, and data acquisition—into a single instrument, making digital PCR faster and easier than ever.
Key Features and Benefits
- Streamlined Workflow:
- qPCR-like workflow with minimal hands-on time (under 5 minutes).
- Fully integrated system for compartmentalization, thermal cycling, and data acquisition.
- Speed and Productivity:
- Complete runs in less than 90 minutes, increasing lab throughput.
- Save time and reagents with multiplexing up to four optical channels.
- High Sensitivity and Accuracy:
- Patented microfluidic array technology ensures over 95% sample utilization across 20,000 microchambers.
- Detect and quantify rare targets with high precision.
- Advanced Software:
- Absolute Q dPCR software simplifies setup, monitoring, and analysis.
- Generate data quickly with adjustable parameters such as plate format and thermal conditions.
- Compliance-ready with features for security, auditing, and e-signatures (21 CFR Part 11 support).
- Multiplexing Capability:
- Measure multiple targets per sample, saving time and increasing experimental efficiency.
- Compatibility with Assays:
- Supports Absolute Q Liquid Biopsy dPCR assays for oncology applications.
- Compatible with TaqMan assays for diverse applications, including gene expression, genetic variation, and gene regulation.
- Smart Remote Support:
- Reduce downtime with immersive remote support tools, minimizing the need for on-site service visits.
Technical Specifications
| Feature | Details |
|---|---|
| Workflow | Single hands-on step (<5 minutes) |
| Run Time | Less than 90 minutes |
| Optical Channels | 4 (Multiplexing enabled) |
| Microfluidic Array | Over 20,000 microchambers per sample |
| Sample Utilization Efficiency | >95% |
| Software Features | Intuitive data analysis, 21 CFR Part 11 compliance support |
| Data Analysis | Export results for downstream analysis or compile into reports |
| Applications | Oncology, gene expression, SNP genotyping, rare target quantification |
| Connectivity | USB, Ethernet |
| Compliance | 21 CFR Part 11 |
| Dimensions | Desktop-friendly design |
| Multiplexing | Yes, up to 4 targets |
Applications
- Oncology Research:
- Liquid biopsy analysis for mutation detection and cancer biomarker quantification.
- Gene Expression Studies:
- Quantify mRNA levels with absolute precision.
- Genetic Variation Analysis:
- Detect SNPs and other genetic mutations.
- Rare Target Quantification:
- Analyze low-abundance targets with high sensitivity.
- Pathogen Detection:
- Identify and quantify viral or bacterial DNA/RNA with precision.
Key Advantages
- Ease of Use: The workflow mimics qPCR simplicity, making digital PCR accessible to all experience levels.
- Speed: Short runtime accelerates experiments without compromising accuracy.
- Precision: High sensitivity and accuracy ensure reliable quantification, even for rare targets.
- Compliance-Ready: Built-in features for regulated environments support data security and auditing.
- Service Excellence: Smart Remote Support minimizes downtime, ensuring smooth lab operations.
The QuantStudio Absolute Q Digital PCR System is ideal for researchers looking for a fast, reliable, and user-friendly dPCR solution that balances performance and simplicity while ensuring compliance with regulated environments.
Comparison with QuantStudio 5, 6 Pro, 7 Pro, and 12K Flex Systems
| Feature | QuantStudio Absolute Q | QuantStudio 5 | QuantStudio 6 Pro | QuantStudio 7 Pro | QuantStudio 12K Flex |
|---|---|---|---|---|---|
| PCR Type | Digital PCR (dPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) |
| Sample Compartmentalization | Integrated microchambers | Not applicable | Not applicable | Not applicable | Not applicable |
| Multiplexing Capability | Up to 4 targets | Up to 6 targets | Up to 5 targets | Up to 6 targets | Up to 4 targets per OpenArray plate |
| Optical Channels | 4 | 6 decoupled (21 filter combinations) | 5 colors (21 filter combinations) | 6 colors (21 filter combinations) | 4 coupled |
| Temperature Control | Single-zone | 6 VeriFlex zones | 3 VeriFlex zones | 6 VeriFlex zones | Standard block |
| Dynamic Range | High sensitivity for low-abundance targets | 10 logs | 10 logs | 10 logs | 9 logs |
| Run Time | <90 minutes | <30 minutes | <30 minutes | <30 minutes | Varies with application |
| Throughput | Moderate | Moderate | Moderate | Moderate | High (up to 12,000 data points per run) |
| Sample Volume Required | ~10–15 µL per reaction | ~20 µL per well | ~20 µL per well | ~20 µL per well | ~2.5 µL per OpenArray reaction |
| Applications | Absolute quantification, rare mutation detection | Gene expression, genotyping | Gene expression, genotyping | Gene expression, genotyping | High-throughput screening |
| Compliance Features | 21 CFR Part 11 support | 21 CFR Part 11 support | 21 CFR Part 11 support | 21 CFR Part 11 support | Not specified |
Comparison with Other Digital PCR Systems
| Feature | QuantStudio Absolute Q | QIAGEN QIAcuity | Bio-Rad QX200 | Stilla Naica System | RainDance RainDrop Plus |
|---|---|---|---|---|---|
| Sample Partitioning | Microchamber array | Nanoplate-based | Droplet-based | Crystal droplet | Droplet-based |
| Hands-On Time | <5 minutes | ~30 minutes | ~15 minutes | ~30 minutes | |
| Run Time | <90 minutes | ~2 hours | ~2.5 hours | ~3 hours | ~2 hours |
| Multiplexing Capability | Up to 4 targets | Up to 5 targets | Up to 2 targets | Up to 6 targets | Up to 2 targets |
| Sample Utilization Efficiency | >95% | ~80% | ~90% | ~80% | |
| Integrated Workflow | Yes | Yes | No (requires separate droplet generator) | Yes | No (requires separate droplet generator) |
| Sample Volume Required | ~10–15 µL per reaction | ~10–15 µL per reaction | ~20 µL per reaction | ~25 µL per reaction | ~20 µL per reaction |
| Applications | Oncology, gene expression, rare mutation detection | Gene expression, rare mutation detection, CNV analysis | Oncology, gene expression, rare mutation detection | Oncology, gene expression, rare mutation detection | Oncology, gene expression, rare mutation detection |
These comparisons highlight the unique features and capabilities of the QuantStudio Absolute Q Digital PCR System relative to other QuantStudio models and competitor digital PCR systems, including the QIAGEN QIAcuity. The Absolute Q system offers a simplified, integrated workflow with high efficiency and multiplexing capabilities, making it a compelling choice for researchers requiring precise and rapid digital PCR analysis.
Absolute Q™ Digital PCR System
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The QuantStudio™ Absolute Q™ Digital PCR (dPCR) System revolutionizes digital PCR by combining the absolute quantification capabilities of dPCR with the simplicity and speed of a qPCR workflow. Designed for researchers who need precise, sensitive, and reproducible quantification, the Absolute Q system integrates all dPCR steps—compartmentalization, thermal cycling, and data acquisition—into a single instrument, making digital PCR faster and easier than ever.
Key Features and Benefits
- Streamlined Workflow:
- qPCR-like workflow with minimal hands-on time (under 5 minutes).
- Fully integrated system for compartmentalization, thermal cycling, and data acquisition.
- Speed and Productivity:
- Complete runs in less than 90 minutes, increasing lab throughput.
- Save time and reagents with multiplexing up to four optical channels.
- High Sensitivity and Accuracy:
- Patented microfluidic array technology ensures over 95% sample utilization across 20,000 microchambers.
- Detect and quantify rare targets with high precision.
- Advanced Software:
- Absolute Q dPCR software simplifies setup, monitoring, and analysis.
- Generate data quickly with adjustable parameters such as plate format and thermal conditions.
- Compliance-ready with features for security, auditing, and e-signatures (21 CFR Part 11 support).
- Multiplexing Capability:
- Measure multiple targets per sample, saving time and increasing experimental efficiency.
- Compatibility with Assays:
- Supports Absolute Q Liquid Biopsy dPCR assays for oncology applications.
- Compatible with TaqMan assays for diverse applications, including gene expression, genetic variation, and gene regulation.
- Smart Remote Support:
- Reduce downtime with immersive remote support tools, minimizing the need for on-site service visits.
Technical Specifications
| Feature | Details |
|---|---|
| Workflow | Single hands-on step (<5 minutes) |
| Run Time | Less than 90 minutes |
| Optical Channels | 4 (Multiplexing enabled) |
| Microfluidic Array | Over 20,000 microchambers per sample |
| Sample Utilization Efficiency | >95% |
| Software Features | Intuitive data analysis, 21 CFR Part 11 compliance support |
| Data Analysis | Export results for downstream analysis or compile into reports |
| Applications | Oncology, gene expression, SNP genotyping, rare target quantification |
| Connectivity | USB, Ethernet |
| Compliance | 21 CFR Part 11 |
| Dimensions | Desktop-friendly design |
| Multiplexing | Yes, up to 4 targets |
Applications
- Oncology Research:
- Liquid biopsy analysis for mutation detection and cancer biomarker quantification.
- Gene Expression Studies:
- Quantify mRNA levels with absolute precision.
- Genetic Variation Analysis:
- Detect SNPs and other genetic mutations.
- Rare Target Quantification:
- Analyze low-abundance targets with high sensitivity.
- Pathogen Detection:
- Identify and quantify viral or bacterial DNA/RNA with precision.
Key Advantages
- Ease of Use: The workflow mimics qPCR simplicity, making digital PCR accessible to all experience levels.
- Speed: Short runtime accelerates experiments without compromising accuracy.
- Precision: High sensitivity and accuracy ensure reliable quantification, even for rare targets.
- Compliance-Ready: Built-in features for regulated environments support data security and auditing.
- Service Excellence: Smart Remote Support minimizes downtime, ensuring smooth lab operations.
The QuantStudio Absolute Q Digital PCR System is ideal for researchers looking for a fast, reliable, and user-friendly dPCR solution that balances performance and simplicity while ensuring compliance with regulated environments.
Comparison with QuantStudio 5, 6 Pro, 7 Pro, and 12K Flex Systems
| Feature | QuantStudio Absolute Q | QuantStudio 5 | QuantStudio 6 Pro | QuantStudio 7 Pro | QuantStudio 12K Flex |
|---|---|---|---|---|---|
| PCR Type | Digital PCR (dPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) | Real-Time PCR (qPCR) |
| Sample Compartmentalization | Integrated microchambers | Not applicable | Not applicable | Not applicable | Not applicable |
| Multiplexing Capability | Up to 4 targets | Up to 6 targets | Up to 5 targets | Up to 6 targets | Up to 4 targets per OpenArray plate |
| Optical Channels | 4 | 6 decoupled (21 filter combinations) | 5 colors (21 filter combinations) | 6 colors (21 filter combinations) | 4 coupled |
| Temperature Control | Single-zone | 6 VeriFlex zones | 3 VeriFlex zones | 6 VeriFlex zones | Standard block |
| Dynamic Range | High sensitivity for low-abundance targets | 10 logs | 10 logs | 10 logs | 9 logs |
| Run Time | <90 minutes | <30 minutes | <30 minutes | <30 minutes | Varies with application |
| Throughput | Moderate | Moderate | Moderate | Moderate | High (up to 12,000 data points per run) |
| Sample Volume Required | ~10–15 µL per reaction | ~20 µL per well | ~20 µL per well | ~20 µL per well | ~2.5 µL per OpenArray reaction |
| Applications | Absolute quantification, rare mutation detection | Gene expression, genotyping | Gene expression, genotyping | Gene expression, genotyping | High-throughput screening |
| Compliance Features | 21 CFR Part 11 support | 21 CFR Part 11 support | 21 CFR Part 11 support | 21 CFR Part 11 support | Not specified |
Comparison with Other Digital PCR Systems
| Feature | QuantStudio Absolute Q | QIAGEN QIAcuity | Bio-Rad QX200 | Stilla Naica System | RainDance RainDrop Plus |
|---|---|---|---|---|---|
| Sample Partitioning | Microchamber array | Nanoplate-based | Droplet-based | Crystal droplet | Droplet-based |
| Hands-On Time | <5 minutes | ~30 minutes | ~15 minutes | ~30 minutes | |
| Run Time | <90 minutes | ~2 hours | ~2.5 hours | ~3 hours | ~2 hours |
| Multiplexing Capability | Up to 4 targets | Up to 5 targets | Up to 2 targets | Up to 6 targets | Up to 2 targets |
| Sample Utilization Efficiency | >95% | ~80% | ~90% | ~80% | |
| Integrated Workflow | Yes | Yes | No (requires separate droplet generator) | Yes | No (requires separate droplet generator) |
| Sample Volume Required | ~10–15 µL per reaction | ~10–15 µL per reaction | ~20 µL per reaction | ~25 µL per reaction | ~20 µL per reaction |
| Applications | Oncology, gene expression, rare mutation detection | Gene expression, rare mutation detection, CNV analysis | Oncology, gene expression, rare mutation detection | Oncology, gene expression, rare mutation detection | Oncology, gene expression, rare mutation detection |
These comparisons highlight the unique features and capabilities of the QuantStudio Absolute Q Digital PCR System relative to other QuantStudio models and competitor digital PCR systems, including the QIAGEN QIAcuity. The Absolute Q system offers a simplified, integrated workflow with high efficiency and multiplexing capabilities, making it a compelling choice for researchers requiring precise and rapid digital PCR analysis.
Absolute Q™ Digital PCR System
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![[A52732] Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit](/web/image/product.product/34499/image_512/%5BA52732%5D%20Applied%20Biosystems%E2%84%A2%20QuantStudio%E2%84%A2%20Absolute%20Q%E2%84%A2%20Digital%20PCR%20Starter%20Kit?unique=d10a093)
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