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Sensitive, fluorescence-based flow cytometry assay for β-galactosidase activity in senescent cells
The Invitrogen™ CellEvent™ Senescence Green Flow Cytometry Assay Kit provides a robust and specific method for detecting cellular senescence in mammalian cells via β-galactosidase hydrolysis. This fixed-cell assay uses a fluorescein-based probe containing two galactoside moieties, making it a precise target for lysosomal β-galactosidase, a well-established biomarker of senescence.
Upon enzymatic cleavage, the probe produces a covalently bound, cell-retained fluorescent signal with excitation/emission maxima of 490/514 nm—readily detectable in the FITC channel of most flow cytometers. The optimized assay buffer ensures reliable staining and minimizes background, while the reagent’s spectral properties enable multi-color compatibility with other fluorescent probes.
Key Features
- Specific Detection – Targets β-galactosidase activity, a hallmark of senescent cells
- Flow Cytometry Optimized – Fixed-cell assay for accurate population analysis
- Bright & Stable Signal – Covalently retained fluorescence for consistent readouts
- Multiplex-Compatible – Works alongside other flow cytometry dyes without significant spectral overlap
- Convenient Kit Format – Includes all reagents and buffers needed for up to 200 assays
Applications
- Quantitative detection of senescent cell populations
- Cancer biology research on tumor suppression and progression
- Aging research and regenerative medicine studies
- Drug screening for compounds modulating senescence
- Multiparametric flow cytometry combining senescence and functional markers
Specifications
| Parameter | Details |
|---|---|
| Catalog Numbers | C10840 (50 assays), C10841 (200 assays) |
| Detection Method | Fluorescence |
| Excitation/Emission | 490/514 nm |
| Format | Fixed-cell assay |
| For Use With | Flow cytometer with FITC detection capability |
| Storage Conditions | Room temperature |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
| Kit Contents | Fluorescein-based β-galactosidase probe and buffer |
The CellEvent™ Senescence Green Flow Cytometry Assay Kit offers high sensitivity, reproducibility, and compatibility for flow cytometric detection of senescent cells. Its design enables quantitative, multi-color analysis in fixed-cell samples, making it an essential tool for senescence research in oncology, aging, and therapeutic development.
Product Sheet: CellEvent Senescence Green Flow Cytometry Assay Kit
Sensitive, fluorescence-based flow cytometry assay for β-galactosidase activity in senescent cells
The Invitrogen™ CellEvent™ Senescence Green Flow Cytometry Assay Kit provides a robust and specific method for detecting cellular senescence in mammalian cells via β-galactosidase hydrolysis. This fixed-cell assay uses a fluorescein-based probe containing two galactoside moieties, making it a precise target for lysosomal β-galactosidase, a well-established biomarker of senescence.
Upon enzymatic cleavage, the probe produces a covalently bound, cell-retained fluorescent signal with excitation/emission maxima of 490/514 nm—readily detectable in the FITC channel of most flow cytometers. The optimized assay buffer ensures reliable staining and minimizes background, while the reagent’s spectral properties enable multi-color compatibility with other fluorescent probes.
Key Features
- Specific Detection – Targets β-galactosidase activity, a hallmark of senescent cells
- Flow Cytometry Optimized – Fixed-cell assay for accurate population analysis
- Bright & Stable Signal – Covalently retained fluorescence for consistent readouts
- Multiplex-Compatible – Works alongside other flow cytometry dyes without significant spectral overlap
- Convenient Kit Format – Includes all reagents and buffers needed for up to 200 assays
Applications
- Quantitative detection of senescent cell populations
- Cancer biology research on tumor suppression and progression
- Aging research and regenerative medicine studies
- Drug screening for compounds modulating senescence
- Multiparametric flow cytometry combining senescence and functional markers
Specifications
| Parameter | Details |
|---|---|
| Catalog Numbers | C10840 (50 assays), C10841 (200 assays) |
| Detection Method | Fluorescence |
| Excitation/Emission | 490/514 nm |
| Format | Fixed-cell assay |
| For Use With | Flow cytometer with FITC detection capability |
| Storage Conditions | Room temperature |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
| Kit Contents | Fluorescein-based β-galactosidase probe and buffer |
The CellEvent™ Senescence Green Flow Cytometry Assay Kit offers high sensitivity, reproducibility, and compatibility for flow cytometric detection of senescent cells. Its design enables quantitative, multi-color analysis in fixed-cell samples, making it an essential tool for senescence research in oncology, aging, and therapeutic development.
Product Sheet: CellEvent Senescence Green Flow Cytometry Assay Kit
![[20688] Thermo Scientific™ Pierce™ Dimethylsulfoxide (DMSO), Sequencing Grade, 950 mL, > 99.5%](/web/image/product.product/40893/image_512/%5B20688%5D%20Thermo%20Scientific%E2%84%A2%20Pierce%E2%84%A2%20Dimethylsulfoxide%20%28DMSO%29%2C%20Sequencing%20Grade%2C%20950%20mL%2C%20%3E%2099.5%25?unique=332c4ff)
![[D12345] Invitrogen™ DMSO, Anhydrous, 3mL](/web/image/product.product/34702/image_512/%5BD12345%5D%20Invitrogen%E2%84%A2%20DMSO%2C%20Anhydrous%2C%203mL?unique=0deaad5)
Specifications
| Pack Size | For 50 Assays, for 200 Assays |