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Invitrogen™ UltraComp eBeads™ Spectral Unmixing Beads, 100 Tests
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | beads | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ UltraComp eBeads™ Spectral Unmixing Beads are third-generation compensation beads designed specifically for spectral flow cytometry. These beads enable precise single-color spectral unmixing and compensation for a wide array of fluorochromes across multiple laser lines, ensuring accurate signal resolution in high-parameter cytometry panels.
Each drop of UltraComp Spectral eBeads contains two bead populations:
- A positive population that binds fluorochrome-conjugated antibodies
- A negative population that does not bind antibodies
This bimodal fluorescence distribution allows researchers to effectively distinguish between bound and unbound beads, providing accurate single-color controls that mimic biological samples more closely than earlier-generation compensation beads.
Key Features and Benefits
- Multispecies Compatibility: Binds antibodies from mouse, rat, hamster, rabbit, and recombinant human sources
- Broad Laser Compatibility: Excitable by UV (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633–640 nm) lasers
- Improved Fluorophore Performance: Optimized for use with modern dyes, reducing spectral spillover and unmixing errors
- Cell-like Unmixing Behavior: Mimics single-color stained cells more accurately in spectral systems
- Reliable Bimodal Distribution: Distinct positive/negative peaks simplify gating and analysis
- Ready-to-use Format: Liquid formulation—no preparation required
Applications
- Spectral flow cytometry
- Single-color controls for spectral unmixing
- Compensation setup in multicolor and high-parameter panels
- Panel validation for immune profiling, oncology, and rare population detection
- Instrument calibration and standardization in spectral systems
Technical Specifications
| Property | Specification |
|---|---|
| Product Type | Compensation / Spectral Unmixing Beads |
| Compatibility | Mouse, rat, hamster, rabbit, recombinant human antibodies |
| Size of Beads | 5 µm |
| Concentration | 5 × 10⁶ beads/mL |
| Excitation Range | UV to IR (355–640 nm lasers) |
| Format | Liquid |
| Storage | 2°C to 8°C (Do not freeze) |
| Shipping | Ice pack |
| Unit Size | 100 tests |
| Regulatory Status | For Research Use Only (RUO) |
UltraComp eBeads™ Spectral Unmixing Beads offer a highly effective solution for researchers working with spectral flow cytometry platforms, providing reliable single-color controls across multiple species and fluorophores. Their advanced formulation ensures low error rates in unmixing, improved fluorochrome compatibility, and a closer match to cellular behavior, making them ideal for high-dimensional panel design and optimization.