CHROMagar™ TBX, for 5L medium

CHROMagar™ TBX is a chromogenic medium specifically designed for the detection and enumeration of ß-glucuronidase positive E. coli in food and animal feed. It provides a clear and efficient method for identifying contamination of fecal origin, aligning with ISO 16649 standards.

Key Features:

1. Chromogenic Detection:
   • Contains a substrate for ß-glucuronidase, allowing E. coli colonies to appear distinctly colored for easy identification.
2. ISO Compliance:
   • Meets the requirements of ISO 16649, the international standard for testing ß-glucuronidase positive E. coli in food and feed.
3. Rapid Results:
   • Enables quick detection and enumeration of E. coli, facilitating timely quality control.
4. Wide Applications:
   • Suitable for use in testing food products and animal feeding stuffs.

Typical Applications:

1. Food Safety Testing:
   • Detection of E. coli in processed and raw foods as an indicator of fecal contamination.
2. Animal Feed Analysis:
   • Identifies contamination in feed that could pose health risks to animals and humans.
3. Quality Assurance:
   • Ensures compliance with food safety standards and regulations.

Preparation Instructions:

1. Dehydrated Medium:
   • Dissolve the entire content of the medium (for 5 L preparation) in distilled water.
   • Heat gently while stirring to dissolve completely.
   • Sterilize by autoclaving at 121°C for 15 minutes.
2. Pouring Plates:
   • Allow the sterilized medium to cool to 45–50°C.
   • Pour into sterile Petri dishes under aseptic conditions.

Testing Procedure:

1. Sample Preparation:
   • Prepare sample dilutions as required.
2. Inoculation:
   • Apply the sample onto the surface of the medium using either the spread plate or pour plate method.
3. Incubation:
   • Incubate the plates at 44°C for 18–24 hours.
4. Interpretation:
   • ß-glucuronidase positive E. coli colonies appear colored, while other organisms are colorless or inhibited.

Advantages:

Storage and Shelf Life:

• Dehydrated Medium:
  • Store at 10–30°C in a cool, dry environment.
  • Shelf life: Check the label for the expiry date.
• Prepared Plates:
  • Store at 2–8°C, away from light, and use within the recommended time frame.

Precautions:

• Handle all samples and plates as potential biohazards.
• Dispose of waste materials according to local and national regulations.

CHROMagar™ TBX offers a reliable and efficient method for detecting and enumerating E. coli, ensuring compliance with safety standards for food and animal feed products.