Apollo Sci. Tris hydrochloride ≥99.5%

TRIS HCl is a widely used buffering agent in biological and biochemical research. This product from Apollo Scientific ensures high purity and reliability for applications requiring strict pH control.

Chemical Overview

• Chemical Name: Tris(hydroxymethyl)aminomethane hydrochloride
• Synonyms:
  • TRIS HCl
  • Tromethamine hydrochloride
  • 2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
  • Trizma® hydrochloride
  • THAM HCl
• Molecular Formula: NH₂C(CH₂OH)₃·HCl
• Molecular Weight: 157.6 g/mol
• CAS Number: 1185-53-1
• EINECS Number: 214-684-5
• MDL Number: MFCD00012590

Physical Properties

• Appearance: White crystalline powder
• Melting Point: 149–153 °C, indicative of its purity and stability.
• pKa @ 20 °C: 8.1, making it an excellent buffer in the pH range of 7.7–9.1.
• Loss on Drying: < 0.5%, demonstrating its low moisture content.
• Heavy Metals Content: < 5 ppm, ensuring minimal contamination.
• Storage Conditions: Ambient temperature storage ensures stability during handling and storage.

Applications

1. Buffer in Biological Systems:
   TRIS HCl is widely used as a buffer in biological and biochemical experiments due to its ability to maintain a stable pH within the useful range of 7.7 to 9.1. Applications include:
   • Preparation of buffer solutions for enzyme activity studies.
   • DNA and RNA isolation procedures.
   • Protein electrophoresis (SDS-PAGE, native PAGE).
   • Cell culture media preparation.
2. Electrophoresis:
   • Used in running buffers for electrophoresis applications like TAE or TBE buffers.
3. Pharmaceutical Applications:
   • Acts as a pH stabilizer in drug formulations.

Chemical Properties

• Buffering Capacity: Strongly buffers biological solutions by dissociating into Tris base and HCl, balancing pH fluctuations in the system.
• Stability: Chemically stable and compatible with many biological reagents and systems.
• Compatibility: Easily soluble in water, making it convenient for preparing solutions.

Safety and Handling

• Hazard Classification: Irritant
• Precautions:
  • Avoid inhalation, ingestion, and contact with skin or eyes.
  • Use appropriate protective equipment (gloves, goggles, and lab coat) during handling.
  • Work in a well-ventilated area or under a fume hood.

Specifications

Storage and Stability

• Storage Temperature: Store at ambient temperature.
• Shelf Life: Stable under recommended storage conditions; prolonged stability in dry environments.

Key Advantages

• High purity (≥99.5%) ensures reliability in sensitive applications.
• Effective buffering range (7.7–9.1) makes it suitable for most biological and biochemical studies.
• Low heavy metal content (< 5 ppm) ensures minimal interference in experimental results.

TRIS HCl from Apollo Scientific is a robust and reliable reagent, tailored for applications requiring precise pH control in biological, chemical, and pharmaceutical fields. Its high purity and excellent buffering properties make it indispensable in laboratories worldwide.