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Thermo Scientific™ Zeba™ Spin Desalting Columns (7K MWCO) are pre-packed, ready-to-use size-exclusion chromatography devices designed for rapid desalting and buffer exchange of protein samples ranging from 2 µL to 20 mL. Featuring a proprietary low-binding resin and a 7,000 Da molecular weight cutoff, these columns effectively remove salts, biotin, fluorescent dyes, and other small molecules, while achieving ≥95% protein recovery.
Zeba columns deliver faster, more consistent results than traditional gravity-flow methods and are ideal for applications such as mass spectrometry, HPLC, capillary electrophoresis, and protein labeling workflows. Available in multiple formats to suit both low-volume and large-scale sample prep, Zeba desalting columns are ideal for high-throughput manual or automated processing.
Key Features & Benefits
-
High Recovery & Low Nonspecific Binding
Proprietary resin ensures excellent recovery of proteins and biological macromolecules. -
Fast, Centrifuge-Based Workflow
Complete desalting or buffer exchange in minutes without lengthy fraction collection. -
Reliable Small Molecule Removal
≥95% removal of salts, free biotin, fluorophores, and other molecules <1,000 Da. -
Flexible Format Options
Available in spin columns (75 µL–20 mL), filter plates (96-well), and chromatography cartridges. -
Ready to Use
Pre-equilibrated and pre-dispensed columns—no packing, washing, or equilibration needed. -
Compatible with Sensitive Workflows
Suitable for protein quantitation, ELISA, Western blot, MS analysis, and enzyme assays.
Available Spin Column Options
| Catalog Number | Quantity | Volume | Sample Volume Range |
|---|---|---|---|
| 89877 | 25 Columns | 75 µL | 2–20 µL |
| 89878 | 50 Columns | 75 µL | 2–20 µL |
| 89882 | 25 Columns | 0.5 mL | 10–130 µL |
| 89883 | 50 Columns | 0.5 mL | 10–130 µL |
| 89889 | 5 Columns | 2 mL | 70–1,000 µL |
| 89890 | 25 Columns | 2 mL | 70–1,000 µL |
| 89891 | 5 Columns | 5 mL | 0.1–1.5 mL |
| 89892 | 25 Columns | 5 mL | 0.1–1.5 mL |
| 89893 | 5 Columns | 10 mL | 0.5–4 mL |
| 89894 | 25 Columns | 10 mL | 0.5–4 mL |
| A40002469 | 5 Columns | 20 mL | 2–10 mL |
| A40002475 | 10 Columns | 20 mL | 2–10 mL |
Applications
-
Mass Spectrometry & HPLC Sample Preparation
Removal of salts, detergents, dyes, and labeling reagents prior to analysis. -
Protein Labeling & Modification Workflows
Buffer exchange prior to or after biotinylation, dye conjugation, or PEGylation. -
Enzyme & Binding Assays
Desalting or buffer switching for improved assay compatibility and sensitivity. -
Antibody Purification & Cleanup
Polishing after affinity purification or conjugation.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO offer a fast, high-recovery alternative to dialysis and gravity-flow columns for protein desalting, buffer exchange, and small molecule removal. Whether you are preparing samples for mass spectrometry, bioconjugation, or functional assays, Zeba columns provide unmatched ease of use, speed, and reproducibility in a variety of volumes and formats to match your experimental needs.
User Guide: Pierce Chromatography Cartridges Desalting
User Guide: Zeba Micro Spin Desalting Columns 7K MWCO
User Guide: Zeba Spin Desalting Plate
User Guide: Zeba XL Spin Desalting Columns, 7K MWCO
Thermo Scientific™ Zeba™ Spin Desalting Columns (7K MWCO) are pre-packed, ready-to-use size-exclusion chromatography devices designed for rapid desalting and buffer exchange of protein samples ranging from 2 µL to 20 mL. Featuring a proprietary low-binding resin and a 7,000 Da molecular weight cutoff, these columns effectively remove salts, biotin, fluorescent dyes, and other small molecules, while achieving ≥95% protein recovery.
Zeba columns deliver faster, more consistent results than traditional gravity-flow methods and are ideal for applications such as mass spectrometry, HPLC, capillary electrophoresis, and protein labeling workflows. Available in multiple formats to suit both low-volume and large-scale sample prep, Zeba desalting columns are ideal for high-throughput manual or automated processing.
Key Features & Benefits
-
High Recovery & Low Nonspecific Binding
Proprietary resin ensures excellent recovery of proteins and biological macromolecules. -
Fast, Centrifuge-Based Workflow
Complete desalting or buffer exchange in minutes without lengthy fraction collection. -
Reliable Small Molecule Removal
≥95% removal of salts, free biotin, fluorophores, and other molecules <1,000 Da. -
Flexible Format Options
Available in spin columns (75 µL–20 mL), filter plates (96-well), and chromatography cartridges. -
Ready to Use
Pre-equilibrated and pre-dispensed columns—no packing, washing, or equilibration needed. -
Compatible with Sensitive Workflows
Suitable for protein quantitation, ELISA, Western blot, MS analysis, and enzyme assays.
Available Spin Column Options
| Catalog Number | Quantity | Volume | Sample Volume Range |
|---|---|---|---|
| 89877 | 25 Columns | 75 µL | 2–20 µL |
| 89878 | 50 Columns | 75 µL | 2–20 µL |
| 89882 | 25 Columns | 0.5 mL | 10–130 µL |
| 89883 | 50 Columns | 0.5 mL | 10–130 µL |
| 89889 | 5 Columns | 2 mL | 70–1,000 µL |
| 89890 | 25 Columns | 2 mL | 70–1,000 µL |
| 89891 | 5 Columns | 5 mL | 0.1–1.5 mL |
| 89892 | 25 Columns | 5 mL | 0.1–1.5 mL |
| 89893 | 5 Columns | 10 mL | 0.5–4 mL |
| 89894 | 25 Columns | 10 mL | 0.5–4 mL |
| A40002469 | 5 Columns | 20 mL | 2–10 mL |
| A40002475 | 10 Columns | 20 mL | 2–10 mL |
Applications
-
Mass Spectrometry & HPLC Sample Preparation
Removal of salts, detergents, dyes, and labeling reagents prior to analysis. -
Protein Labeling & Modification Workflows
Buffer exchange prior to or after biotinylation, dye conjugation, or PEGylation. -
Enzyme & Binding Assays
Desalting or buffer switching for improved assay compatibility and sensitivity. -
Antibody Purification & Cleanup
Polishing after affinity purification or conjugation.
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO offer a fast, high-recovery alternative to dialysis and gravity-flow columns for protein desalting, buffer exchange, and small molecule removal. Whether you are preparing samples for mass spectrometry, bioconjugation, or functional assays, Zeba columns provide unmatched ease of use, speed, and reproducibility in a variety of volumes and formats to match your experimental needs.
User Guide: Pierce Chromatography Cartridges Desalting
User Guide: Zeba Micro Spin Desalting Columns 7K MWCO
User Guide: Zeba Spin Desalting Plate
User Guide: Zeba XL Spin Desalting Columns, 7K MWCO
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Specifications
| Capacity | 2 mL, 25 Columns, 5 mL, 25 Columns, 10 mL, 25 Columns, 75 μL, 25 Columns, 75 μL, 50 Columns, 20 mL, 5 Columns, 20 mL, 10 Columns, 5 mL, 5 Columns, 10 mL, 5 Columns, 2 mL, 5 Columns, 0.5 mL, 25 Columns, 0.5 mL, 50 Columns |