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Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a high-purity, 10% (w/v) nonionic surfactant, specially formulated for reliable performance in ELISA, Western blotting, and immunoassays. Packaged under nitrogen gas in glass ampules or non-leaching HDPE bottles, this solution ensures long-term stability, eliminates peroxide accumulation, and prevents degradation.
Tween™ 20 is widely used in biochemical, molecular biology, and pharmaceutical research due to its low toxicity, excellent biocompatibility, and strong protein-stabilizing properties. This detergent plays a key role in reducing non-specific binding in assays, improving wash buffers, and solubilizing membrane proteins.
Key Features & Benefits
- High-Purity 10% (w/v) Tween™ 20 Detergent Solution
- Precisely formulated to ensure batch-to-batch consistency.
- Stable nonionic surfactant suitable for sensitive biological applications.
- Enhanced Stability & Purity
- Packaged under nitrogen gas to prevent oxidation and degradation.
- Contains <1.0 μeq/mL peroxides and carbonyls, ensuring high purity.
- Ideal for Immunoassays & Molecular Biology
- Optimized for ELISA, Western blot, and immunoassay wash buffers.
- Reduces background noise in antibody-based assays.
- Versatile Applications in Protein & Lipid Research
- Effective in membrane protein extraction and stabilization.
- Used in cell lysis, protein solubilization, and lipid-based assays.
- Convenient & Ready-to-Use Format
- Available in glass ampules or HDPE bottles for precise dispensing.
- Easily diluted to desired working concentrations.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | Tween™ 20 Surfact-Amps™ Detergent Solution |
| Catalog Numbers | 85113 (50 mL), 28320 (6 × 10 mL), 85114 (250 mL), 85115 (500 mL), 28321 (1 L) |
| Concentration | 10% (w/v) Tween™ 20 in ultrapure water |
| Molecular Weight | 1228 g/mol |
| Form | Liquid |
| Detergent Class | Nonionic polyoxyethylene surfactant |
| Cloud Point | 95°C |
| Critical Micelle Concentration (CMC) | 0.06 mM (0.0074%, w/v) |
| Oxidants | ≤1.0 μeq/mL |
| Carbonyls | ≤1.0 μeq/mL |
| Suspended Solids | Residue must not exceed Residue Reference |
| Dialyzable | No |
| Storage Conditions | Store in a cool, dry, well-ventilated area |
| Shipping Condition | Room Temperature |
| Reagent Type | Detergent Solution |
| Unit Size | Each |
Use Cases & Applications
1. Immunoassays & Antibody-Based Assays
- Blocking & Wash Buffer Additive in ELISA & Western Blot
- Reduces non-specific binding to increase assay sensitivity.
- Used in PBS and TBS buffers for consistent signal detection.
- Immunohistochemistry & Flow Cytometry Applications
- Ensures optimal antigen-antibody interactions.
- Minimizes background interference in fluorescence-based assays.
2. Protein Extraction & Stabilization
- Membrane Protein Solubilization & Isolation
- Enhances protein extraction without denaturation.
- Used in detergent-based cell lysis buffers.
- Enzyme Stabilization & Protein Purification
- Prevents protein aggregation in chromatography & purification processes.
- Ideal for enzymatic assays requiring nonionic detergents.
3. Molecular Biology & Genomics
- DNA & RNA Extraction Protocols
- Used in nucleic acid purification buffers for cell lysis and protein removal.
- Ensures RNA integrity in sensitive extraction protocols.
- PCR & qPCR Reaction Optimization
- Enhances reaction efficiency by reducing surface tension.
- Compatible with Taq polymerase-based amplification methods.
4. Cell Culture & Drug Delivery Research
- Detergent for Cell Membrane Permeabilization
- Facilitates antibody staining and intracellular protein detection.
- Commonly used in cell-based permeability assays.
- Surfactant in Liposome & Nanoparticle Formulations
- Supports liposome stabilization in drug delivery systems.
- Used in biocompatible emulsions and surfactant-stabilized formulations.
5. Industrial & Biotechnological Applications
- Emulsifier in Food & Beverage Processing
- Acts as a stabilizer in emulsions and food formulations.
- Ensures consistent texture and shelf-life improvement.
- Cosmetic & Personal Care Formulations
- Used in lotions, shampoos, and skincare products for emulsion stability.
- Provides mild surfactant properties in cleansing formulations.
Usage & Handling Instructions
Preparation & Dilution:
- For Immunoassay Buffers (ELISA, Western Blot):
- Dilute Tween™ 20 to 0.05–0.1% (v/v) in PBS or TBS buffer.
- For Protein Solubilization & Lysis Buffers:
- Use at 0.1–2% (w/v) depending on application.
- For Cell Culture & Permeabilization:
- Use at 0.01–0.5% (w/v) for mild permeabilization.
Handling Precautions:
- Avoid prolonged exposure to air, as Tween™ 20 can degrade over time.
- Work in a well-ventilated environment when preparing solutions.
- Always use RNase/DNase-free conditions when working with nucleic acid extractions.
Storage Recommendations:
- Keep at room temperature in a dry, well-ventilated area.
- Protect from direct sunlight and heat to prevent degradation.
- Ensure cap is tightly sealed after each use to maintain reagent integrity.
Disposal Guidelines:
- Dispose of according to local biosafety and chemical waste disposal regulations.
- Avoid direct disposal into drains or water sources without proper neutralization.
Why Choose Thermo Scientific™ Tween™ 20 Surfact-Amps™?
✅ High-purity 10% Tween™ 20 solution for reproducible results
✅ Packaged under nitrogen gas to prevent peroxides and degradation
✅ Ready-to-use format for ELISA, Western blot, and cell culture applications
✅ Nonionic surfactant with excellent protein-stabilizing properties
✅ Compatible with immunoassays, molecular biology, and biopharmaceutical research
Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a reliable, high-purity surfactant designed for consistent and reproducible results in immunoassays, molecular biology, and industrial applications.
No resources are currently available for this product.
Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a high-purity, 10% (w/v) nonionic surfactant, specially formulated for reliable performance in ELISA, Western blotting, and immunoassays. Packaged under nitrogen gas in glass ampules or non-leaching HDPE bottles, this solution ensures long-term stability, eliminates peroxide accumulation, and prevents degradation.
Tween™ 20 is widely used in biochemical, molecular biology, and pharmaceutical research due to its low toxicity, excellent biocompatibility, and strong protein-stabilizing properties. This detergent plays a key role in reducing non-specific binding in assays, improving wash buffers, and solubilizing membrane proteins.
Key Features & Benefits
- High-Purity 10% (w/v) Tween™ 20 Detergent Solution
- Precisely formulated to ensure batch-to-batch consistency.
- Stable nonionic surfactant suitable for sensitive biological applications.
- Enhanced Stability & Purity
- Packaged under nitrogen gas to prevent oxidation and degradation.
- Contains <1.0 μeq/mL peroxides and carbonyls, ensuring high purity.
- Ideal for Immunoassays & Molecular Biology
- Optimized for ELISA, Western blot, and immunoassay wash buffers.
- Reduces background noise in antibody-based assays.
- Versatile Applications in Protein & Lipid Research
- Effective in membrane protein extraction and stabilization.
- Used in cell lysis, protein solubilization, and lipid-based assays.
- Convenient & Ready-to-Use Format
- Available in glass ampules or HDPE bottles for precise dispensing.
- Easily diluted to desired working concentrations.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | Tween™ 20 Surfact-Amps™ Detergent Solution |
| Catalog Numbers | 85113 (50 mL), 28320 (6 × 10 mL), 85114 (250 mL), 85115 (500 mL), 28321 (1 L) |
| Concentration | 10% (w/v) Tween™ 20 in ultrapure water |
| Molecular Weight | 1228 g/mol |
| Form | Liquid |
| Detergent Class | Nonionic polyoxyethylene surfactant |
| Cloud Point | 95°C |
| Critical Micelle Concentration (CMC) | 0.06 mM (0.0074%, w/v) |
| Oxidants | ≤1.0 μeq/mL |
| Carbonyls | ≤1.0 μeq/mL |
| Suspended Solids | Residue must not exceed Residue Reference |
| Dialyzable | No |
| Storage Conditions | Store in a cool, dry, well-ventilated area |
| Shipping Condition | Room Temperature |
| Reagent Type | Detergent Solution |
| Unit Size | Each |
Use Cases & Applications
1. Immunoassays & Antibody-Based Assays
- Blocking & Wash Buffer Additive in ELISA & Western Blot
- Reduces non-specific binding to increase assay sensitivity.
- Used in PBS and TBS buffers for consistent signal detection.
- Immunohistochemistry & Flow Cytometry Applications
- Ensures optimal antigen-antibody interactions.
- Minimizes background interference in fluorescence-based assays.
2. Protein Extraction & Stabilization
- Membrane Protein Solubilization & Isolation
- Enhances protein extraction without denaturation.
- Used in detergent-based cell lysis buffers.
- Enzyme Stabilization & Protein Purification
- Prevents protein aggregation in chromatography & purification processes.
- Ideal for enzymatic assays requiring nonionic detergents.
3. Molecular Biology & Genomics
- DNA & RNA Extraction Protocols
- Used in nucleic acid purification buffers for cell lysis and protein removal.
- Ensures RNA integrity in sensitive extraction protocols.
- PCR & qPCR Reaction Optimization
- Enhances reaction efficiency by reducing surface tension.
- Compatible with Taq polymerase-based amplification methods.
4. Cell Culture & Drug Delivery Research
- Detergent for Cell Membrane Permeabilization
- Facilitates antibody staining and intracellular protein detection.
- Commonly used in cell-based permeability assays.
- Surfactant in Liposome & Nanoparticle Formulations
- Supports liposome stabilization in drug delivery systems.
- Used in biocompatible emulsions and surfactant-stabilized formulations.
5. Industrial & Biotechnological Applications
- Emulsifier in Food & Beverage Processing
- Acts as a stabilizer in emulsions and food formulations.
- Ensures consistent texture and shelf-life improvement.
- Cosmetic & Personal Care Formulations
- Used in lotions, shampoos, and skincare products for emulsion stability.
- Provides mild surfactant properties in cleansing formulations.
Usage & Handling Instructions
Preparation & Dilution:
- For Immunoassay Buffers (ELISA, Western Blot):
- Dilute Tween™ 20 to 0.05–0.1% (v/v) in PBS or TBS buffer.
- For Protein Solubilization & Lysis Buffers:
- Use at 0.1–2% (w/v) depending on application.
- For Cell Culture & Permeabilization:
- Use at 0.01–0.5% (w/v) for mild permeabilization.
Handling Precautions:
- Avoid prolonged exposure to air, as Tween™ 20 can degrade over time.
- Work in a well-ventilated environment when preparing solutions.
- Always use RNase/DNase-free conditions when working with nucleic acid extractions.
Storage Recommendations:
- Keep at room temperature in a dry, well-ventilated area.
- Protect from direct sunlight and heat to prevent degradation.
- Ensure cap is tightly sealed after each use to maintain reagent integrity.
Disposal Guidelines:
- Dispose of according to local biosafety and chemical waste disposal regulations.
- Avoid direct disposal into drains or water sources without proper neutralization.
Why Choose Thermo Scientific™ Tween™ 20 Surfact-Amps™?
✅ High-purity 10% Tween™ 20 solution for reproducible results
✅ Packaged under nitrogen gas to prevent peroxides and degradation
✅ Ready-to-use format for ELISA, Western blot, and cell culture applications
✅ Nonionic surfactant with excellent protein-stabilizing properties
✅ Compatible with immunoassays, molecular biology, and biopharmaceutical research
Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a reliable, high-purity surfactant designed for consistent and reproducible results in immunoassays, molecular biology, and industrial applications.
No resources are currently available for this product.
Specifications
| Pack Size | 1L, 50 mL, 500 mL, 250 mL, 6 x 10 mL |