Thermo Scientific™ Tween 20 Washing Buffer, 2% in PBS (20X)

Thermo Scientific™ Tween 20 Washing Buffer, 2% in PBS (20X) is a concentrated phosphate-buffered saline detergent solution formulated for highly effective washing and blocking in immunoassays and immunolabeling workflows. This PBS-T buffer contains an elevated concentration of 2% Tween 20, a gentle nonionic surfactant that enhances washing performance, reduces nonspecific background staining, and improves reagent spreading in automated staining systems.

Supplied as a convenient 20X stock concentrate, this solution is ideal for laboratories requiring a space-saving, ready-to-dilute buffer for routine preparation of working-strength PBS-T wash buffers.

Key Features and Benefits

Enhanced Background Reduction with 2% Tween 20

The high detergent concentration improves removal of unbound antibodies and proteins, significantly reducing nonspecific staining and background noise.

Optimized for Immunolabeling and Automated Staining

Tween 20 promotes uniform reagent spreading, making this buffer suitable for automated immunostaining methodologies.

Ideal for Immunoassay Washing and Blocking

Commonly used in:

• ELISA plate washing
• Western blot membrane washing
• General immunoassay protocols
• Plate-based blocking applications

Convenient 20X Concentrated Format

Space-saving stock solution that allows rapid dilution to 1X working buffer, ensuring consistent preparation and reduced handling time.

Ambient Storage Convenience

Recommended for storage at ambient temperatures, simplifying laboratory logistics.

Applications

Thermo Scientific™ PBS-T (2% Tween 20, 20X) is widely used for:

• Wash buffer preparation for ELISA assays
• Western blot washing steps
• Immunolabeling and immunostaining protocols
• Automated staining reagent spreading
• Reduction of nonspecific background staining
• Blocking buffer for plate-based assays

Product Specifications

Ordering Information

Thermo Scientific™ Tween 20 Washing Buffer, 2% in PBS (20X), is a robust, high-strength PBS-T solution designed for laboratories performing sensitive immunoassays and immunolabeling workflows. With enhanced detergent content for superior washing efficiency, reduced background staining, and improved reagent spreading, it is an essential reagent for reproducible ELISA, Western blotting, and automated staining applications. The convenient 20X concentrate format ensures efficient preparation and space-saving storage.