Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X)

Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X) is a high-quality concentrated wash buffer designed for routine and sensitive immunodetection workflows. Commonly referred to as PBS-T, this buffer combines phosphate-buffered saline with the nonionic detergent Tween 20, providing excellent washing performance while maintaining stable pH conditions.

It is widely used as a wash solution for Western blot membranes, ELISA microtiter plate wells, and other antibody-based assay systems. The optimized formulation effectively removes unbound proteins, antibodies, and excess reagents, reducing background signal and improving assay clarity.

Supplied as a convenient 10X concentrate, this solution supports efficient preparation of working-strength wash buffer for daily laboratory use.

Key Features and Benefits

Optimized for Western Blot and ELISA Washing

PBS-T with Tween 20 is a standard wash buffer in immunoassays, helping improve signal-to-noise ratio by reducing nonspecific binding.

Effective Removal of Excess Material

The detergent component enhances washing efficiency by removing residual antibodies, proteins, and contaminants from membranes and plate surfaces.

Stable pH and Ionic Strength

Phosphate-buffered saline provides consistent buffering capacity and salt composition, supporting reliable assay performance.

Convenient Concentrated Format

Delivered as a 10X solution, allowing easy dilution to 1X working concentration and reducing preparation time.

Suitable for Biomedical and Laboratory Research

Used extensively in diagnostic development, protein analysis, and general laboratory detergent applications.

Applications

Thermo Scientific™ Tween 20 Washing Buffer is commonly used for:

• Western blot membrane washing steps
• ELISA microplate washing
• Immunoassay protocols requiring low background
• Removal of unbound antibodies and reagents
• General laboratory detergent and biomedical research workflows

Product Specifications

Ordering Information

Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X), is a reliable and convenient concentrated wash solution for Western blotting, ELISA, and other immunoassay workflows. Its optimized PBS-detergent formulation ensures effective removal of excess materials, reduced nonspecific binding, and consistent assay performance. An essential reagent for molecular biology and biomedical laboratories requiring reproducible immunodetection results.