Thermo Scientific™ TRIS-Buffered Saline (TBS, 20X) with 2% Tween 20

Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 2% Tween 20 is a concentrated, high-performance wash buffer designed for demanding immunoassay and protein detection workflows. Commonly referred to as TBST, this solution combines the buffering capacity of Tris-saline with an enhanced concentration of the nonionic detergent Tween 20, providing superior reduction of nonspecific binding and protein–protein interactions.

This buffer is widely used for washing steps in Western blotting, ELISA, and general immunodetection assays, as well as for sample preparation and dilution of secondary antibodies or streptavidin conjugates.

Supplied as a convenient ready-to-use 20X concentrate, it allows rapid preparation of working-strength wash buffer while ensuring reproducible results.

Key Features and Benefits

Enhanced Background Reduction with 2% Tween 20

The increased detergent content helps minimize nonspecific antibody binding and improves washing efficiency in sensitive immunoassays.

Ideal for Washing and Sample Preparation

Used extensively as a wash buffer between immunoreaction steps and for preparing antibody or conjugate incubation solutions.

Stable Tris-Saline Buffer System

TBS provides consistent ionic strength and pH stability, supporting optimal antigen–antibody interactions.

Convenient Concentrated Format

Delivered as a 20X stock solution, reducing preparation time and ensuring easy dilution to 1X working concentration.

Water Soluble and Easy to Handle

Fully soluble in water and suitable for routine laboratory workflows.

Research Use Only

Intended for laboratory research applications (RUO).

Applications

Thermo Scientific™ TBS (20X) with 2% Tween 20 is suitable for:

• Western blot membrane washing
• ELISA and immunoassay wash steps
• Reduction of nonspecific binding in antibody assays
• Dilution of secondary antibodies
• Preparation of streptavidin-AP conjugate solutions
• General immunodetection sample preparation workflows

Formulation Notes

• 1.0 M Tris-HCl
• 3.0 M NaCl
• pH 7.4 ± 0.1
• 2.0% Tween 20

Product Specifications

Ordering Information

Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 2% Tween 20 is a robust and highly effective TBST wash buffer for immunoassay workflows requiring enhanced detergent strength. By reducing nonspecific binding and improving washing efficiency, it supports clearer results and higher assay reproducibility in Western blotting, ELISA, and antibody-based detection protocols. Its convenient concentrated format makes it an essential reagent for modern research laboratories.