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Pierce™ Quantitative Peptide Assays are robust, mix-and-read microplate-based assays for sensitive and reproducible quantitation of peptide concentrations—ideal for LC/MS, LC-MS/MS, and other mass spectrometry workflows. The kits come in fluorescence or colorimetric detection formats, each including a validated peptide digest reference standard for reliable standard curve generation and normalization of experimental samples.
These assays are designed to overcome common limitations of traditional protein assays (e.g., BCA, Bradford) when applied to peptide mixtures, providing superior linearity, specificity, and compatibility with mass spectrometry reagents, including TMT-labels (colorimetric only).
Key Features & Benefits
| Feature | Fluorescent Assay (23290) | Colorimetric Assay (23275) |
|---|---|---|
| Detection Method | Fluorescence (Ex/Em: 390/475 nm) | Absorbance (480 nm) |
| Sensitivity | 5–1000 µg/mL | 25–1000 µg/mL |
| Sample Volume | 10 µL | 20 µL |
| Readout Time | 5 minutes | 15 minutes |
| TMT-Compatible | No | Yes |
| Suitable for Synthetic Peptides | Yes (if free amino-terminus) | Not recommended (signal depends on peptide composition) |
| Standard Included | Yes – Peptide Digest Assay Standard (1 mg/mL, 1.5 mL) | Yes – Peptide Digest Assay Standard (1 mg/mL, 1.5 mL) |
| Reproducibility | High – minimal lot-to-lot variation | High – tested for digest consistency |
Applications
-
Mass Spectrometry Sample Preparation
- Normalize sample concentrations for LC-MS/MS injection.
- Ensure consistent peptide loading across conditions.
-
Peptide Cleanup Efficiency
- Evaluate recovery post desalting or SPE.
-
Proteomics Research
- Assess tryptic digestion efficiency or quantitate peptide synthesis.
-
Assay Development
- Standardize peptide input in immunoassays or ELISA development.
Contents
Catalog No. 23290 – Fluorescent Peptide Assay Kit
- Fluorescent Peptide Assay Reagent: 4 × 2.5 mL
- Fluorescent Peptide Assay Buffer: 50 mL
- Peptide Digest Assay Standard (1 mg/mL): 1.5 mL
- Storage: 2–8°C
Catalog No. 23275 – Colorimetric Peptide Assay Kit
- Colorimettric Assay Reagent A, 50 mL
- Colorimetric Assay Reagent B, 2 x 25 mL
- Colorimetric Assay Reagent C, 2 mL
- Peptide Digest Assay Standard, 1.5 mL
- Storage: 2–8°C
Catalog No. 23295 – Peptide Digest Assay Standard Only
- Ready-to-use reference standard for use with 23275 or 23290
- Digestion verified with MS-grade trypsin
- Supplied as 1 mg/mL in 1.5 mL volume
- Storage: 2–8°C
The Pierce™ Quantitative Peptide Assays & Standards offer a high-performance, MS-compatible solution for reproducible peptide quantitation with minimal setup. Whether you need rapid fluorescence-based detection or TMT-compatible colorimetric assays, these kits streamline the standardization of peptide concentrations for proteomics workflows, improving accuracy and throughput.
User Guide: Pierce Quantitative Colorimetric Peptide Assay Kit
User Guide: Pierce Quantitative Fluorometric Peptide Assay Kit
Pierce™ Quantitative Peptide Assays are robust, mix-and-read microplate-based assays for sensitive and reproducible quantitation of peptide concentrations—ideal for LC/MS, LC-MS/MS, and other mass spectrometry workflows. The kits come in fluorescence or colorimetric detection formats, each including a validated peptide digest reference standard for reliable standard curve generation and normalization of experimental samples.
These assays are designed to overcome common limitations of traditional protein assays (e.g., BCA, Bradford) when applied to peptide mixtures, providing superior linearity, specificity, and compatibility with mass spectrometry reagents, including TMT-labels (colorimetric only).
Key Features & Benefits
| Feature | Fluorescent Assay (23290) | Colorimetric Assay (23275) |
|---|---|---|
| Detection Method | Fluorescence (Ex/Em: 390/475 nm) | Absorbance (480 nm) |
| Sensitivity | 5–1000 µg/mL | 25–1000 µg/mL |
| Sample Volume | 10 µL | 20 µL |
| Readout Time | 5 minutes | 15 minutes |
| TMT-Compatible | No | Yes |
| Suitable for Synthetic Peptides | Yes (if free amino-terminus) | Not recommended (signal depends on peptide composition) |
| Standard Included | Yes – Peptide Digest Assay Standard (1 mg/mL, 1.5 mL) | Yes – Peptide Digest Assay Standard (1 mg/mL, 1.5 mL) |
| Reproducibility | High – minimal lot-to-lot variation | High – tested for digest consistency |
Applications
-
Mass Spectrometry Sample Preparation
- Normalize sample concentrations for LC-MS/MS injection.
- Ensure consistent peptide loading across conditions.
-
Peptide Cleanup Efficiency
- Evaluate recovery post desalting or SPE.
-
Proteomics Research
- Assess tryptic digestion efficiency or quantitate peptide synthesis.
-
Assay Development
- Standardize peptide input in immunoassays or ELISA development.
Contents
Catalog No. 23290 – Fluorescent Peptide Assay Kit
- Fluorescent Peptide Assay Reagent: 4 × 2.5 mL
- Fluorescent Peptide Assay Buffer: 50 mL
- Peptide Digest Assay Standard (1 mg/mL): 1.5 mL
- Storage: 2–8°C
Catalog No. 23275 – Colorimetric Peptide Assay Kit
- Colorimettric Assay Reagent A, 50 mL
- Colorimetric Assay Reagent B, 2 x 25 mL
- Colorimetric Assay Reagent C, 2 mL
- Peptide Digest Assay Standard, 1.5 mL
- Storage: 2–8°C
Catalog No. 23295 – Peptide Digest Assay Standard Only
- Ready-to-use reference standard for use with 23275 or 23290
- Digestion verified with MS-grade trypsin
- Supplied as 1 mg/mL in 1.5 mL volume
- Storage: 2–8°C
The Pierce™ Quantitative Peptide Assays & Standards offer a high-performance, MS-compatible solution for reproducible peptide quantitation with minimal setup. Whether you need rapid fluorescence-based detection or TMT-compatible colorimetric assays, these kits streamline the standardization of peptide concentrations for proteomics workflows, improving accuracy and throughput.
User Guide: Pierce Quantitative Colorimetric Peptide Assay Kit
User Guide: Pierce Quantitative Fluorometric Peptide Assay Kit
![[88378] Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay (Black)](/web/image/product.product/43607/image_512/%5B88378%5D%20Thermo%20Scientific%E2%84%A2%2096-Well%20Plates%20for%20Pierce%E2%84%A2%20Quantitative%20Fluorometric%20Peptide%20Assay%20%28Black%29%20?unique=96c5da2)
Specifications
| Type | Peptide Assay for Fluorescence detection, Peptide Assay for Colorimetric detection, Peptide Digest Assay Standard only |