Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a high-efficiency, ready-to-use solution designed for fast and reliable first-strand cDNA synthesis in two-step RT-qPCR workflows. Available with or without a genomic DNA (gDNA) removal step using dsDNase, this kit enables rapid, high-yield synthesis of full-length cDNA up to 20 kb from RNA templates ranging from as low as 1 pg up to 5 µg. The use of Maxima Reverse Transcriptase, engineered for superior thermostability and reaction kinetics, ensures enhanced performance across a broad range of RNA inputs and temperatures (42–65°C).

Key Features and Benefits

• 🔹 Efficient First-Strand cDNA Synthesis: Yields full-length cDNA up to 20 kb with high sensitivity and specificity.
• 🔹 Rapid Workflow: Reaction complete within 15–30 minutes; streamlined setup with pre-mixed reagents.
• 🔹 Broad Compatibility: Effective with a wide range of RNA inputs (1 pg–5 µg).
• 🔹 High Thermostability: Enables efficient cDNA synthesis even in high-GC or structured templates.
• 🔹 Integrated gDNA Removal (in dsDNase kit): Novel double-strand-specific DNase removes contaminating gDNA without degrading RNA.

Kit Components

Applications

• Two-step RT-qPCR (quantitative real-time PCR)
• Two-step RT-PCR (conventional endpoint PCR)
• Expression profiling and transcript quantification
• cDNA synthesis from degraded, low-concentration, or structured RNA templates

Specifications

The Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR offers an optimized, high-performance solution for generating reliable cDNA for quantitative PCR. With rapid processing, high yield, and an option for integrated genomic DNA removal, this kit supports diverse molecular biology workflows in research, gene expression analysis, and transcriptome studies with precision and convenience.