Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

The Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit is a high-performance reverse transcription system designed for first-strand cDNA synthesis from RNA templates. It features the Maxima H Minus Reverse Transcriptase, a highly thermostable enzyme derived from MMLV RT via in vitro evolution. This enzyme is RNase H-deficient, allowing for synthesis of long, full-length cDNAs up to 20 kb, even from complex or GC-rich templates.

The kit is available in two configurations:

• Standard version (K1651 & K1652)
• With dsDNase (K1681 & K1682) – for integrated genomic DNA removal in a simplified one-tube workflow.

Key Features & Benefits

✅ High Efficiency & Thermostability

• Synthesizes full-length cDNA from RNA templates up to 20 kb
• Operates efficiently at 42°C to 65°C to overcome secondary RNA structures
• Reaction completed in just 30 minutes

✅ No RNase H Activity

• RNase H-minus (H–) reverse transcriptase ensures longer, intact cDNA synthesis by preventing degradation of RNA templates during extension.

✅ Flexible Priming Options

• Compatible with:
  • Oligo(dT)18 primers (selective for mRNA)
  • Random hexamer primers (for total RNA)
  • Gene-specific primers (for targeted RT)

✅ Optional dsDNase Integration (K1681/K1682)

• Removes contaminating genomic DNA from RNA in 2 minutes, using a highly specific double-stranded DNase
• Enables one-tube gDNA removal + cDNA synthesis workflow

✅ Complete Kit with Quality Reagents

• Includes:
  • Maxima H Minus Enzyme Mix (RT + RiboLock RNase Inhibitor)
  • Oligo(dT)18 and Random Hexamer Primers
  • 5X RT Buffer
  • dNTP Mix
  • Nuclease-free water
  • dsDNase & 10X dsDNase buffer (in dsDNase versions)

Ordering Options & Formats

Applications

• RT-PCR and qRT-PCR (Real-Time PCR)
• cDNA Library Construction
• Gene Expression Analysis
• Antisense RNA Synthesis
• Probe Generation for Hybridization

Specifications

Why Choose Maxima H Minus First Strand cDNA Synthesis Kit?

✅ High yields of full-length cDNA even from long or structured RNA templates

✅ Flexible priming strategies for total RNA or mRNA

✅ One-tube workflow with genomic DNA removal (dsDNase version)

✅ Fast reaction time—30 minutes to cDNA

✅ Proven performance in qPCR, RT-PCR, and gene expression analysis

Whether you need a robust reverse transcription system for routine use or demanding applications, the Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit provides superior performance, convenience, and flexibility.