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Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a water-soluble, amine-reactive biotinylation reagent designed for efficient, selective labeling of proteins, antibodies, and other amine-containing macromolecules. With a pegylated spacer arm consisting of four ethylene glycol units, this reagent offers enhanced solubility and reduced steric hindrance—making it ideal for sensitive bioconjugation workflows that require high stability and improved target accessibility.
Key Features and Benefits
-
Efficient Amine Reactivity:
- Contains an NHS (N-hydroxysuccinimide) ester group that forms stable, irreversible amide bonds with primary amines on lysine residues or protein N-termini.
-
Hydrophilic PEG Spacer (4 units, 29 Å):
- Reduces steric interference during streptavidin binding.
- Enhances aqueous solubility of the biotinylated product and minimizes aggregation, especially in stored antibodies.
-
Cell-Impermeable:
- Ideal for surface labeling applications, such as labeling of cell surface proteins or extracellular domains, due to its inability to cross intact membranes.
-
Stable & Versatile:
- Suitable for proteins, antibodies, and peptide conjugates.
- Can be used in aqueous buffer systems or dissolved in DMSO, DMF, or water.
Applications
-
Biotinylation of antibodies for:
- Western blotting
- Immunohistochemistry (IHC)
- ELISA detection
- Flow cytometry
- Immobilization of proteins for affinity purification on streptavidin or avidin supports.
- Surface labeling of membrane proteins without cell permeation.
- Enhancing solubility and reducing aggregation of protein conjugates in solution.
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | A39259 (10 × 2 mg), 21330 (25 mg), 21362 (50 mg), 21363 (1 g) |
| Reactive Group | NHS ester |
| Target Functional Group | Primary amines (-NH₂) |
| Spacer Arm | PEG4 (29.0 Å) |
| Solubility | Water, DMSO, DMF |
| Cell Permeability | No (cell-impermeant) |
| Label Type | Biotin |
| Formulation | Solid |
| Storage Conditions | Store desiccated at –20°C |
| Shipping Condition | Wet ice |
| Quantity | Multiple pack sizes available |
EZ-Link™ NHS-PEG4-Biotin is a premium reagent designed for optimal protein labeling, balancing accessibility, stability, and solubility. Its pegylated spacer arm makes it particularly suitable for labeling targets where steric hindrance can compromise detection sensitivity or capture efficiency. Whether used in antibody conjugation, surface biotinylation, or affinity purification workflows, this reagent ensures consistent and high-performance results in biotin-streptavidin based detection and separation systems.
User Guide: EZ-Link NHS-PEG4-Biotin
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a water-soluble, amine-reactive biotinylation reagent designed for efficient, selective labeling of proteins, antibodies, and other amine-containing macromolecules. With a pegylated spacer arm consisting of four ethylene glycol units, this reagent offers enhanced solubility and reduced steric hindrance—making it ideal for sensitive bioconjugation workflows that require high stability and improved target accessibility.
Key Features and Benefits
-
Efficient Amine Reactivity:
- Contains an NHS (N-hydroxysuccinimide) ester group that forms stable, irreversible amide bonds with primary amines on lysine residues or protein N-termini.
-
Hydrophilic PEG Spacer (4 units, 29 Å):
- Reduces steric interference during streptavidin binding.
- Enhances aqueous solubility of the biotinylated product and minimizes aggregation, especially in stored antibodies.
-
Cell-Impermeable:
- Ideal for surface labeling applications, such as labeling of cell surface proteins or extracellular domains, due to its inability to cross intact membranes.
-
Stable & Versatile:
- Suitable for proteins, antibodies, and peptide conjugates.
- Can be used in aqueous buffer systems or dissolved in DMSO, DMF, or water.
Applications
-
Biotinylation of antibodies for:
- Western blotting
- Immunohistochemistry (IHC)
- ELISA detection
- Flow cytometry
- Immobilization of proteins for affinity purification on streptavidin or avidin supports.
- Surface labeling of membrane proteins without cell permeation.
- Enhancing solubility and reducing aggregation of protein conjugates in solution.
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | A39259 (10 × 2 mg), 21330 (25 mg), 21362 (50 mg), 21363 (1 g) |
| Reactive Group | NHS ester |
| Target Functional Group | Primary amines (-NH₂) |
| Spacer Arm | PEG4 (29.0 Å) |
| Solubility | Water, DMSO, DMF |
| Cell Permeability | No (cell-impermeant) |
| Label Type | Biotin |
| Formulation | Solid |
| Storage Conditions | Store desiccated at –20°C |
| Shipping Condition | Wet ice |
| Quantity | Multiple pack sizes available |
EZ-Link™ NHS-PEG4-Biotin is a premium reagent designed for optimal protein labeling, balancing accessibility, stability, and solubility. Its pegylated spacer arm makes it particularly suitable for labeling targets where steric hindrance can compromise detection sensitivity or capture efficiency. Whether used in antibody conjugation, surface biotinylation, or affinity purification workflows, this reagent ensures consistent and high-performance results in biotin-streptavidin based detection and separation systems.
User Guide: EZ-Link NHS-PEG4-Biotin
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Specifications
| Pack Size | 25 mg, 50 mg, Chloroacetamide (10 x 2 mg), 1 g |