Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kit

Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kits provide a complete, optimized solution for fluorescent labeling of small quantities of antibody or protein (100 μg per reaction) with DyLight™ NHS ester fluorophores. These microscale kits are ideal for research workflows that require multiple small-scale labeling experiments for applications such as immunofluorescence microscopy, flow cytometry, Western blotting, ELISA, and IHC.

Each kit contains pre-measured, single-use vials of amine-reactive DyLight dye, as well as all necessary buffers, purification resin, and spin columns. Labeled antibodies are typically ready in 90 minutes with minimal hands-on time and are suitable for direct use in fluorescence-based detection assays.

Key Features and Benefits

Optimized for Small-Scale Labeling

• Designed to label 100 μg of IgG or protein per reaction.
• Perform up to 5 labeling reactions per kit with consistent results.

Bright, Stable Fluorescent Signal

• DyLight dyes provide high fluorescence intensity and superior photostability across pH 4–9.
• Comparable to or brighter than CyDye and LI-COR™ dyes in most applications.

Reliable Amine-Reactive Chemistry

• NHS-ester-activated dyes form stable amide bonds with primary amines on lysine residues or protein N-termini.
• Specific labeling with minimal nonspecific modification.

Convenient All-in-One Kit Format

• Includes pre-aliquoted dye vials, borate buffer, purification resin, spin columns, and collection tubes.
• No need to weigh reagents or source additional components.

Applications

• Immunofluorescence microscopy (IF)
• Flow cytometry and cell surface staining
• Immunohistochemistry (IHC)
• Western blot detection
• ELISA fluorescence detection
• In vitro and in vivo protein labeling

Technical Specifications

Available DyLight™ Microscale Kits

DyLight™ Microscale Antibody Labeling Kits offer a flexible and efficient approach for fluorescently labeling small quantities of antibodies or proteins. Ideal for research labs needing high-performance fluorophores in microscale formats, these kits deliver reproducible, bright conjugates ready for immediate use in fluorescence-based applications.