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Bromophenol Blue (BPB) is a pH indicator and tracking dye commonly used in biochemical assays, gel electrophoresis, and acid-base titrations. Supplied in ACS reagent-grade purity by Thermo Fisher Scientific, this compound exhibits a distinct visual transition from yellow to blue between pH 3.0 and 4.6. Its strong colorimetric response and compatibility with aqueous systems make it a staple reagent in protein and nucleic acid research.
Bromophenol Blue is often used to monitor sample migration during electrophoresis and can serve as a visual endpoint marker in pH-sensitive reactions and tests.
Key Features
- ACS-grade reagent for analytical and biochemical applications
- pH transition interval: pH 3.0 (yellow) to pH 4.6 (blue)
- Color varies from pink-orange to red to purple in solid form
- High-purity powder with confirmed infrared spectrum and clarity test
- Stable at ambient temperatures
- Excellent solubility in aqueous and some polar solvents
Chemical & Physical Properties
| Property | Specification |
|---|---|
| Appearance | Pink-orange to red to purple powder |
| Melting Point | 279 °C |
| Density (20 °C) | 1.0 g/cm³ |
| pH Transition Range | pH 3.0 (yellow) to pH 4.6 (blue) |
| Infrared Spectrum | Conforms |
| Clarity of Solution | Passes test |
| Solubility | Soluble in water and alcohols |
| Stability | Stable under ambient storage |
Packaging Options
| Catalog # | Pack Size | Packaging Type | |
|---|---|---|---|
| 403140050 | 5 g | Glass bottle for solids | |
| 403140100 | 10 g | Glass bottle for solids | |
| 403140500 | 50 g | Glass bottle |
Applications
- pH indicator in analytical titrations
- Tracking dye in agarose and polyacrylamide gel electrophoresis
- Sample loading dye in SDS-PAGE and nucleic acid gels
- Indicator in protein quantification (e.g., Bradford assay)
- Colorimetric detection in acid-base reaction monitoring
- pH control and buffering system validation
Thermo Fisher Scientific Bromophenol Blue ACS offers a high-purity, reliable reagent for biochemical research, electrophoresis, and acid-base analytical methods. With a sharp visual pH transition and excellent stability, it serves as a trusted indicator and migration marker for routine laboratory and research
No resources are currently available for this product.
Bromophenol Blue (BPB) is a pH indicator and tracking dye commonly used in biochemical assays, gel electrophoresis, and acid-base titrations. Supplied in ACS reagent-grade purity by Thermo Fisher Scientific, this compound exhibits a distinct visual transition from yellow to blue between pH 3.0 and 4.6. Its strong colorimetric response and compatibility with aqueous systems make it a staple reagent in protein and nucleic acid research.
Bromophenol Blue is often used to monitor sample migration during electrophoresis and can serve as a visual endpoint marker in pH-sensitive reactions and tests.
Key Features
- ACS-grade reagent for analytical and biochemical applications
- pH transition interval: pH 3.0 (yellow) to pH 4.6 (blue)
- Color varies from pink-orange to red to purple in solid form
- High-purity powder with confirmed infrared spectrum and clarity test
- Stable at ambient temperatures
- Excellent solubility in aqueous and some polar solvents
Chemical & Physical Properties
| Property | Specification |
|---|---|
| Appearance | Pink-orange to red to purple powder |
| Melting Point | 279 °C |
| Density (20 °C) | 1.0 g/cm³ |
| pH Transition Range | pH 3.0 (yellow) to pH 4.6 (blue) |
| Infrared Spectrum | Conforms |
| Clarity of Solution | Passes test |
| Solubility | Soluble in water and alcohols |
| Stability | Stable under ambient storage |
Packaging Options
| Catalog # | Pack Size | Packaging Type | |
|---|---|---|---|
| 403140050 | 5 g | Glass bottle for solids | |
| 403140100 | 10 g | Glass bottle for solids | |
| 403140500 | 50 g | Glass bottle |
Applications
- pH indicator in analytical titrations
- Tracking dye in agarose and polyacrylamide gel electrophoresis
- Sample loading dye in SDS-PAGE and nucleic acid gels
- Indicator in protein quantification (e.g., Bradford assay)
- Colorimetric detection in acid-base reaction monitoring
- pH control and buffering system validation
Thermo Fisher Scientific Bromophenol Blue ACS offers a high-purity, reliable reagent for biochemical research, electrophoresis, and acid-base analytical methods. With a sharp visual pH transition and excellent stability, it serves as a trusted indicator and migration marker for routine laboratory and research
No resources are currently available for this product.
Specifications
| Pack Size | 5g, 10g, 50g |