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Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO
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Specifications:
| Application | Size Exclusion Chromatography |
| Storage Temperature | 2-8°C |
| Product Type | columns |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Zeba™ Spin Desalting Columns with 40K MWCO are pre-packed, centrifuge-ready tools designed for the rapid and efficient removal of low-molecular-weight contaminants such as salts, dyes, crosslinkers, and other small molecules (<2 kDa) from high-molecular-weight proteins and complexes (>40 kDa). These columns are powered by a proprietary size-exclusion chromatography resin that delivers superior flow characteristics and outstanding protein recovery, even at low concentrations.
Designed for exceptional versatility, Zeba 40K MWCO spin columns are available in a broad range of formats, accommodating sample volumes from 5 µL up to 10 mL. This makes them ideal for workflows ranging from small-scale protein analysis to high-volume buffer exchange.
Key Features & Benefits
-
High-Molecular-Weight Compatibility
Specifically designed for protein and macromolecular desalting where molecular weights exceed 40 kDa. -
Efficient Removal of Small Contaminants
Eliminates >95% of low molecular weight components including salts, buffer additives, unreacted dyes, and excess reagents. -
Maximized Protein Recovery
Proprietary resin minimizes sample loss and maintains protein integrity, even at dilute concentrations. -
Fast Workflow in Centrifuge Format
No fraction collection, equilibration, or chromatography system required—just spin and recover. -
Flexible Volumes & Formats
Available for a variety of scales and applications: micro spin, standard spin, and high-capacity spin column formats. -
Ready to Use
Pre-packed and pre-hydrated columns for immediate use—no resin hydration or column packing necessary.
Applications
- Buffer exchange before chromatography, electrophoresis, or enzymatic reactions
- Cleanup after protein labeling or modification
- Sample preparation prior to mass spectrometry (MS) or HPLC
- Removal of excess reagents such as NHS esters, biotin, fluorescent tags, or reducing agents
- Desalting of antibodies, enzymes, or large protein complexes
Technical Specifications
| Feature | Details |
|---|---|
| Product Type | Spin Desalting Column |
| Molecular Weight Cut-Off | 40,000 Da |
| Resin Type | Proprietary size-exclusion chromatography |
| Format | Micro spin, standard spin, high-capacity |
| Sample Volume Range | 5 µL – 10 mL (model dependent) |
| Compatibility | Centrifuge-based purification |
| Target Molecules | Proteins and biomolecules >40 kDa |
| Removal Range | Removes molecules <2 kDa |
| Use | Single-use disposable |
| Storage Conditions | 4–8°C |
| Product Line | Zeba™ |
| Catalog Number | Quantity | Sample Volume Range |
|---|---|---|
| A57756 | 25 columns | 5–14 µL |
| A57758 | 50 columns | 5–14 µL |
| A57759 | 25 columns | 50–150 µL |
| A57760 | 50 columns | 50–150 µL |
| A57761 | 5 columns | 300–800 µL |
| A57762 | 25 columns | 300–800 µL |
| A57763 | 5 columns | 500–2000 µL |
| A57764 | 25 columns | 500–2000 µL |
| A57765 | 5 columns | 1000–4000 µL |
| A57766 | 25 columns | 1000–4000 µL |
| A40002471 | 5 columns | 4–10 mL |
| A40002476 | 10 columns | 4–10 mL |
The Zeba™ Spin Desalting Columns, 40K MWCO are a robust and flexible solution for laboratories requiring rapid buffer exchange or cleanup of high-molecular-weight proteins. Their ready-to-use format, high-performance resin, and compatibility with standard centrifuges make them an indispensable tool in protein purification workflows. Whether you're preparing samples for mass spectrometry, chromatography, or biochemical assays, Zeba columns offer efficient, reliable, and reproducible results, saving both time and resources in the lab.
- Sample volume: 50 to 150 μL, 50 columns 4 to 10 mL, 5 Columns 4 to 10 mL, 10 Columns 5 to 14 μL, 25 columns 5 to 14 μL, 50 columns 50 to 150 μL, 25 columns 300 to 800 μL, 5 columns 300 to 800 μL, 25 columns 500 to 2000 μL, 5 columns 500 to 2000 μL, 25 columns 1000 to 4000 μL, 5 columns 1000 to 4000 μL, 25 columns