Thermo Scientific™ Water, nuclease-free
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CAS Number :
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Formula: H₂O
CAS Number: 7732-18-5
Specifications:
Application | molecular biology | ||
Storage Temperature | Ambient | ||
Product Type | Molecular Biology Reagent | Forms | Liquid |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
Thermo Scientific™ Nuclease-Free Water is specially prepared for molecular biology applications, offering high purity and the assurance of being free from nucleases and phosphatases. This water is deionized and membrane-filtered through a 0.22 µm filter, making it ideal for sensitive techniques such as PCR, RT-PCR, qPCR, RNA and DNA extraction, and other enzymatic reactions. It is not DEPC-treated, reducing potential enzyme inhibition in downstream applications.
Key Features
- Nuclease-Free – Free from RNases, DNases, and phosphatases
- High Purity – Deionized and 0.22 µm membrane-filtered
- Molecular Biology Grade – Suitable for all nucleic acid workflows
- Convenient Volumes – Available in small aliquots and bulk formats
- Not DEPC-Treated – Minimizes interference with enzyme activity
Specifications
Property | Specification |
---|---|
Product Name | Nuclease-Free Water |
Purity Level | Free of endo-/exo-DNase, RNase, phosphatase |
Filtration | 0.22 µm membrane-filtered |
Treatment | Not DEPC-treated |
Grade | Molecular Biology |
pH | 7.6 |
Packaging Type | Vial |
Unit Size | Each |
Applications
- PCR, RT-PCR, qPCR – Ideal for amplifying nucleic acids without enzyme degradation
- RNA & DNA Extraction – Maintains integrity during sample preparation
- Enzymatic Reactions – For cloning, restriction digestion, ligation, and labeling
- Library Preparation – Suitable for sequencing and transcriptomics workflows
- General Molecular Biology – Compatible with all standard protocols requiring nuclease-free conditions
Ordering Information
Catalog Number | Volume | Packaging Type |
---|---|---|
R0581 | 4 × 1.25 mL | Vial |
R0582 | 30 mL | Vial |
Thermo Scientific™ Nuclease-Free Water provides dependable purity and enzyme safety for a wide range of molecular biology procedures. With strict quality control and optimized packaging, it is a reliable choice for researchers requiring contamination-free aqueous solutions.
- Pack Size: 30 mL 4 x 1.25 mL