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Thermo Scientific™ TRITON™ X-100, 100mL
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Detergent, equivalent to Nonidet P-40
Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
TRITON™ X-100 is a mild, non-ionic surfactant and emulsifier optimized for biochemical and molecular biology applications. With proven non-denaturing properties, TRITON™ X-100 allows the gentle solubilization of membrane proteins, cell lysis, and membrane extraction without disrupting protein structure or enzymatic activity.
This surfactant is ideal for immunohistochemistry (IHC), immunofluorescence (IF), Western blotting, flow cytometry, and a variety of enzyme activity assays. Tested for RNase and DNase, it is suitable for nucleic acid and protein applications that demand high purity and reproducibility.
Key Features & Benefits
-
Mild, Non-Denaturing Detergent
Preserves native protein conformation and function. -
Versatile Application Range
Compatible with IHC, IF, FACS, Western blot, and biochemical assays. -
High Purity for Molecular Workflows
RNase/DNase-free; safe for sensitive nucleic acid and protein research. -
Cell Permeabilization for Intracellular Access
Enables effective intracellular staining in immunoassays and flow cytometry. -
Reliable Surfactant for Consistency
Supports reproducible results in solubilization, staining, and enzymatic protocols.
Common Applications & Use Cases
| Application Area | Use Case |
|---|---|
| Protein Solubilization | Lysis of mammalian cells to extract cytosolic and membrane-bound proteins. |
| Immunohistochemistry (IHC) | Permeabilization of tissue sections for enhanced antibody staining. |
| Immunofluorescence (IF) | Flat-mount staining of retina or brain tissue for high-resolution imaging. |
| Flow Cytometry (FACS) | Selective permeabilization of apoptotic cells for Annexin V and LDH assays. |
| Membrane Protein Isolation | Extraction of outer membrane proteins from bacterial cultures. |
| Biochemical Enzyme Assays | Estimation of lipase and LDH activity in plasma or tissue lysates. |
Technical Specifications
| Feature | Description |
|---|---|
| Product Name | TRITON™ X-100 |
| Chemical Type | Non-ionic surfactant |
| Form | Liquid |
| Biochemical Properties | Mild, non-denaturing detergent |
| Compatibility | IHC, IF, FACS, Western blot, biochemical assays |
| RNase/DNase Tested | Yes |
| Storage Conditions | Room temperature |
| Solubility | Water and buffer systems |
Why Choose TRITON™ X-100?
- Gentle on Proteins – Maintains structural integrity during extraction.
- Ideal for IHC & Imaging – Enhances antibody penetration and signal clarity.
- Broad Compatibility – Versatile across molecular and cellular workflows.
- Cell-Friendly – Enables precise intracellular access for flow cytometry and microscopy.
- Reliable & High Purity – Trusted in academic, clinical, and industrial labs worldwide.
The TRITON™ X-100 is a trusted non-ionic surfactant used extensively for membrane protein extraction, immunodetection techniques, cell-based assays, and biochemical enzyme analysis. Its gentle, non-denaturing nature makes it indispensable for researchers aiming to preserve protein functionality while ensuring experimental consistency in high-sensitivity assays.