Thermo Scientific™ Pierce™ C18 Spin Tips

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Specifications:

Application	Size Exclusion Chromatography, Mass Spectrometry
Storage Temperature	2-8°C
Product Type	Sample Prep
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The Thermo Scientific™ Pierce™ C18 Spin Tips and Spin Columns are high-efficiency, ready-to-use tools for the desalting, cleanup, and enrichment of peptides prior to mass spectrometry (MS) analysis. They employ a porous reverse-phase C18 resin or monolithic sorbent, enabling the rapid and reproducible removal of interfering substances such as salts, detergents, and other MS-incompatible compounds. Designed for flexibility, the format supports a wide range of peptide sample volumes and concentrations, ensuring excellent peptide recovery and improved spectral quality for MALDI-MS and ESI-MS.

Key Features & Benefits

High Recovery & Sensitivity
Retains and elutes peptides efficiently—even from sub-picomole quantities of digested protein.
Rapid & Convenient Sample Prep
Complete cleanup and elution in under five minutes using a simple 5-step spin protocol.
MS-Compatible Output
Peptides are eluted in solvents suitable for LC-MS/MS or MALDI-MS, free from salts or detergents.
Flexible Volumes & Formats
Available in both spin tip and pipette tip formats for manual or automated workflows.
Centrifuge-Compatible Design
Spin tips include adaptors for easy microcentrifuge processing without special equipment.
Low Dead Volume & Minimal Loss
Engineered to prevent sample loss during loading or elution, ensuring maximum peptide recovery.

Applications

Peptide desalting and sample prep for MALDI-MS or LC-MS/MS
Cleanup of peptide mixtures post-enzymatic digestion
Concentration of dilute peptide solutions
Removal of buffers, salts, and surfactants before MS analysis
High-throughput proteomics workflows and biomarker discovery

Technical Specifications

Parameter	Details
Product Type	Spin Tip / Pipette Tip / Spin Column
Sorbent	C18 Reverse-Phase Resin / Monolithic C18
Binding Capacity	Up to 10 μg peptides per 20 μL sample
Volume Range	10 – 100 μL (Spin Tips)
Format	Spin Tips (84850), 10 μL Tips (87782), 100 μL Tips (87784)
Sample Type	Protease-digested proteins, peptides
Compatible Equipment	Microcentrifuge, Mass Spectrometer
Workflow Step	Sample Clean-up, Peptide Desalting
Detection Method	Mass Spectrometry (MALDI-MS, ESI-MS)
Storage Conditions	Room temperature

The Pierce™ C18 Spin Tips and Spin Columns offer an efficient and reproducible method to clean up peptide samples before mass spectrometry. Their user-friendly design, high recovery rate, and compatibility with common MS platforms make them a staple in proteomics, biomarker validation, and protein characterization workflows.