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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Phusion™ High-Fidelity DNA Polymerase is a robust and highly accurate enzyme designed for PCR applications requiring exceptional fidelity, speed, and yield. Its error rate is 52 times lower than Taq and 6 times lower than Pfu, making it ideal for cloning, sequencing, mutagenesis, and other precision-demanding applications. Available in colorless and green buffer formats, it offers flexibility for downstream analysis.
Key Features:
- Unparalleled Fidelity:
- 52× higher fidelity than Taq DNA polymerase for applications requiring precision.
- Exceptional Speed:
- Short extension times of 15–30 seconds per kilobase for rapid amplification.
- High Yield and Robust Performance:
- Generates high yields even with minimal enzyme amounts.
- Performs well even in challenging conditions (e.g., GC-rich templates or PCR inhibitors).
- Convenient Formats:
- Colorless Buffer: For standard PCR applications.
- Green Buffer: Includes a loading dye for direct gel electrophoresis.
- Versatile Applications:
- Supports a range of applications from high-fidelity PCR and cloning to long-range PCR (up to 20 kb).
Available Formats and Catalog Numbers:
| Catalog Number | Buffer Type | No. of Reactions | Format |
|---|---|---|---|
| F530S | Colorless | 100 Reactions | Standard |
| F530L | Colorless | 500 Reactions | Standard |
| F530XL | Colorless | 2000 Reactions | Bulk |
| F534S | Green | 100 Reactions | Direct-Load |
| F534L | Green | 500 Reactions | Direct-Load |
Specifications:
| Attribute | Details |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Concentration | 2 U/μL |
| Reaction Speed | Fast (15–30 s/kb) |
| Fidelity | 52× Taq |
| Error Rate | 50-fold lower than Taq; 6-fold lower than Pfu |
| PCR Size Range | Up to 20 kb |
| Applications | Standard PCR, high-fidelity PCR, mutagenesis, sequencing |
| GC-Rich Templates | High performance |
| Overhang | Blunt |
| Buffers Included | Phusion HF & GC Buffers with 1.5 mM MgCl₂ in 1X |
| DMSO Included | Yes |
| Shipping Conditions | Dry Ice |
| Storage | -20°C |
Kit Contents:
- Phusion DNA Polymerase (2 U/μL).
- 5X Phusion HF Buffer: For high fidelity.
- 5X Phusion GC Buffer: Optimized for GC-rich templates.
- 50 mM MgCl₂ solution.
- DMSO: For enhanced performance with challenging templates.
Applications:
- High-Fidelity PCR:
- Ideal for applications requiring minimal sequence errors, such as cloning and sequencing.
- Template Amplification:
- Amplifies difficult GC-rich or long templates up to 20 kb.
- Mutagenesis:
- Suitable for site-directed mutagenesis.
- High-Throughput PCR:
- Consistent and reproducible results for automated workflows.
- Microarray and Sequencing:
- Generates templates for downstream genomic analysis.
Advantages:
| Feature | Benefit |
|---|---|
| Unmatched Fidelity | Reduced errors for highly accurate results. |
| Fast Reaction Times | Short PCR protocols save time. |
| Robust in Challenging PCR | Effective with inhibitors and GC-rich regions. |
| High Yield | Minimal enzyme use reduces costs. |
| Flexible Formats | Options for direct gel loading or standard PCR. |
Handling and Storage:
- Store at -20°C to maintain enzyme activity.
- Avoid repeated freeze-thaw cycles to ensure consistent performance.
- Reactions: 100 rxn 500 rxn 2000 rxn
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