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Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Format: 2X Master Mix | Polymerase: Phusion Flash II DNA Polymerase
Storage: –20 °C | Shipping: Dry Ice
Use: For research use only (RUO)
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix is an advanced, ready-to-use PCR solution designed for ultra-fast, high-accuracy DNA amplification. Utilizing a proprietary hot-start Phusion Flash II DNA Polymerase, this 2X master mix enables extension times as short as 15 seconds per kilobase while maintaining superior fidelity—25 times higher than Taq DNA polymerase.
Ideal for time-sensitive and complex workflows, this formulation supports a range of applications including GC-rich templates, cloning, mutagenesis, and multiplex PCR.
Key Features
- Extreme reaction speed: Extension time as fast as 15 s/kb
- Built-in hot-start functionality: Allows immediate reaction setup and zero-time reactivation
- High fidelity: 25X more accurate than Taq polymerase for error-sensitive applications
- Blunt-end PCR product: Ideal for high-accuracy cloning and downstream processing
- Ready-to-use: 2X format minimizes pipetting and contamination risk
- High yield performance: Suitable for both low- and high-complexity templates
- Wide range of applications: From fast routine PCR to long-range and high-GC templates
Specifications
| Parameter | Details |
|---|---|
| Master Mix Format | 2X concentration |
| Color | Colorless |
| Polymerase | Phusion Flash II DNA Polymerase |
| Fidelity (vs. Taq) | 25X |
| Reaction Speed | Very fast (≤15 s/kb) |
| PCR Product End Type | Blunt |
| GC-Rich Template Support | High |
| Final Product Size | Up to 20 kb |
| Hot Start | Built-in |
| No. of Reactions (F548S) | 100 |
| No. of Reactions (F548L) | 500 |
| Storage | –20 °C |
| Shipping Conditions | On dry ice |
| For Use With Applications | High-fidelity PCR, fast PCR, cloning, mutagenesis, sequencing prep, multiplex PCR |
Applications
- High-speed PCR for rapid workflows
- High-fidelity amplification for cloning, sequencing, or diagnostics
- Difficult templates such as GC-rich or complex regions
- Template preparation for next-gen sequencing
- Multiplex PCR for multiple targets in a single reaction
- Long-range PCR for amplicons up to 20 kb
- Mutagenesis and site-directed editing applications
- Microarray-based sample prep
Notes
- Phusion DNA polymerase annealing rules differ from standard Taq-based systems. Primer Tm should be calculated using tools optimized for high-fidelity polymerases.
- Designed for research use only—not intended for diagnostic or clinical applications.
Ordering Information
| Catalog # | Description | Reactions | Storage |
|---|---|---|---|
| F548S | Phusion Flash High-Fidelity PCR Master Mix | 100 | –20 °C |
| F548L | Phusion Flash High-Fidelity PCR Master Mix | 500 | –20 °C |
Thermo Scientific™ Phusion Flash Master Mix is the ideal solution for researchers who need fast, high-fidelity PCR with minimal optimization. Its robust enzyme formulation, compatibility with complex templates, and low error rate make it a top choice for modern molecular biology workflows.
- Pack Size: 1 x 1 mL, sufficient for 100 Rxns 5 x 1 mL, Sufficient for 500 Rxns