Thermo Scientific™ PCR Master Mix (2X)

The Thermo Scientific PCR Master Mix is a ready-to-use 2X solution containing Taq DNA Polymerase, dNTPs, and optimized PCR components. This all-in-one formulation streamlines the PCR setup process, minimizing pipetting steps, reducing contamination risk, and ensuring reproducible results for routine PCR amplification of DNA fragments up to 5 kb.

Key Features:

1. Convenient Pre-Mixed Formulation:
   • Simplifies PCR setup by including Taq DNA Polymerase, dNTPs, and reaction buffers in a single 2X mix.
   • Requires only the addition of DNA template and primers.
2. Thermostability:
   • Taq DNA Polymerase has a half-life of over 40 minutes at 95°C, ensuring robust performance during PCR cycling.
3. Reproducible Results:
   • Optimized for consistent amplification of DNA fragments up to 5 kb.
4. 3'-dA Overhang Products:
   • PCR products generated with Taq DNA Polymerase are suitable for TA cloning.
5. Labeling Capabilities:
   • Compatible with the incorporation of modified nucleotides, such as biotin-, digoxigenin-, or fluorescently-labeled nucleotides.

Applications:

1. Routine DNA Amplification:
   • Ideal for standard PCR of DNA fragments up to 5 kb.
2. High-Throughput PCR:
   • Suitable for applications requiring high efficiency and reproducibility.
3. DNA Labeling:
   • Enables labeling of PCR products with modified nucleotides for downstream applications.

Specifications:

Available Formats and Catalog Numbers:

Kit Contents:

1. 2X PCR Master Mix:
   • 4 × 1.25 mL for the 200-reaction format.
2. Nuclease-Free Water:
   • 4 × 1.25 mL for dilution and reaction setup.

Performance Metrics:

• Error Rate: 2.2 × 10⁻⁵ errors/nt/cycle.
• Accuracy: 4.5 × 10⁴ (inverse of error rate; represents average number of correct nucleotides incorporated before an error occurs).

Advantages:

Handling and Storage:

• Store at -20°C to maintain enzyme activity.
• Avoid repeated freeze-thaw cycles for best results.