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Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated)
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Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated) is a selective and differential medium designed for the detection, enumeration, and differentiation of Escherichia coli and coliforms through single membrane filtration. This medium is ideal for monitoring fecal contamination in drinking water and assessing water treatment efficacy.
Key Features
- Selective and Differential Medium:
- Lauryl sulphate selectively inhibits Gram-positive organisms, allowing coliforms to grow.
- The combination of lactose and BCIG (5-bromo-4-chloro-3-indolyl-β-D-glucuronide) facilitates differentiation of E. coli and other coliforms.
- Clear Differentiation:
- E. coli colonies appear green due to lactose fermentation and glucuronidase activity.
- Other coliforms appear yellow, indicating lactose fermentation without glucuronidase activity.
- Enhanced Specificity:
- Reduces the need for confirmatory tests for E. coli due to the specificity of the reactions within the medium.
- Ideal for Water Testing:
- Supports single membrane filtration for monitoring fecal contamination in water supplies.
Applications
1. Water Microbiology
- Detection of E. coli and coliforms in drinking water and treated water systems.
2. Environmental Monitoring
- Monitoring fecal contamination in recreational water and industrial effluents.
3. Public Health Compliance
- Ensures water quality meets safety and regulatory standards for public health.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Membrane Lactose Glucuronide Agar (MLGA) |
| Applications | Water microbiology and environmental testing |
| Quantity | 500g |
| Shelf Life | 3 years |
| Storage Conditions | 10–30°C |
Preparation Instructions
- Suspend: Suspend 36g of MLGA powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- Precise Differentiation: Distinguishes E. coli (green colonies) from other coliforms (yellow colonies).
- Enhanced Specificity: Reduces confirmatory testing for E. coli, saving time and resources.
- Regulatory Compliance: Suitable for monitoring water quality to meet public health standards.
- Ease of Use: Supports single membrane filtration for efficient testing.
- Cost-Effective: High yield of 500g, suitable for routine laboratory testing.
Diagnostic Features
| Organism | Reaction |
|---|---|
| Escherichia coli | Green colonies (lactose fermentation + glucuronidase activity) |
| Other coliforms | Yellow colonies (lactose fermentation only) |
| Non-Coliforms | No growth or inhibited |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: 3 years from the date of manufacture.
Packaging
- Product: Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated)
- Catalog Numbers:
- CM1031B: 500g
- CM1031T: 5kg
The Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) is a trusted solution for the detection, enumeration, and differentiation of E. coli and coliforms in water samples. Its selective and differential properties, coupled with single membrane filtration compatibility, make it essential for laboratories monitoring water safety, environmental quality, and public health compliance.
- Pack Size: 500g 5kg