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Thermo Scientific™ Nigrosine, water soluble
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Specifications:
| Application | Hematology | ||
| Storage Temperature | Ambient | ||
| Product Type | Biological Stain | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₂₂H₁₄N₆Na₂O₉S₂ |
Thermo Scientific™ Nigrosin (water soluble) is a black synthetic dye supplied in crystalline or powdered form, widely used as a biological stain, industrial dye, and pigment additive in both research and manufacturing applications. With strong absorbance in the 560–590 nm range (UV-Vis, water), it is an effective negative stain and coloring agent across biological, textile, polymer, and printing sectors.
This formulation is water soluble and suitable for a broad range of solubility profiles, including ethanol, oil, and fat-based systems. Nigrosin is used as a negative stain for observing spirochetes, bacteria, protozoa, and fungi in microbiological preparations, and as a biological counterstain in cell and tissue imaging. It is also commonly found in inks, plastics, leather dyes, phenolic resins, and printing applications.
Key Features and Benefits
- Research-Grade Quality: Certified for research use with high specific absorbance (>200 E 1%/1cm at λmax)
- Effective Biological Stain: Commonly used in Dorner's spore stain and as a negative stain for microbial observation
- Multifunctional Industrial Dye: Used in phenolic resins, printing inks, textiles, plastics, and more
- Solubility Versatility: Soluble in water, ethanol, oil, and fat—ideal for diverse formulations
- Legacy Alfa Aesar Quality: Maintains original specifications under the Thermo Scientific Chemicals brand
Chemical and Physical Properties
| Property | Description |
|---|---|
| Product Name | Nigrosin, Water Soluble |
| CAS Number | 8005-03-6 |
| Molecular Formula | C₂₂H₁₄N₆Na₂O₉S₂ |
| Molecular Weight | 616.49 g/mol |
| IUPAC Name | Disodium (3E)-5-amino-6-[(1E)-2-(4-nitrophenyl)diazen-1-yl]-4-oxo-3-(2-phenylhydrazin-1-ylidene)-3,4-dihydronaphthalene-2,7-disulfonate |
| Appearance | Black crystals or powder |
| λmax (UV-Vis, water) | 560–590 nm |
| Specific Absorbance | >200 E 1%/1cm at λmax |
| Loss on Drying | ≤15% at 105°C (constant weight) |
| Solubility | Soluble in water, ethanol, oil, and fats |
| Storage | Cool, dry, well-ventilated area; keep tightly closed |
| Usage Classification | RUO – Research Use Only |
Available Catalog Numbers and Packaging
| Catalog Number | Quantity | Packaging |
|---|---|---|
| A18147.14 | 25 g | Glass bottle |
| A18147.22 | 100 g | Glass bottle |
Applications
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Biological Staining:
- Negative staining for spirochetes, bacteria, protozoa, and fungi
- Dorner’s method for spore staining
- Live/dead cell differentiation in microscopy
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Industrial Uses:
- Dyeing of leather, wood, textiles, and plastics
- Manufacturing of shoe polish and inks
- Coloring phenolic, polyamide, styrenic, and urea resins
- Printing color bases
Thermo Scientific™ Nigrosin, Water Soluble, is a versatile black dye ideal for both biological and industrial applications. Its high solubility and broad staining capabilities make it a valuable tool for microbiologists, histologists, and formulation chemists alike. Whether used as a negative stain in microbiological analysis or as a coloring agent in polymers and resins, Nigrosin delivers consistency and performance. Available in 25 g and 100 g pack sizes, it supports both routine and scale-up use across research and production environments.
- Pack Size: 100g 25g