Thermo Scientific™ N-Lauroylsarcosine sodium salt, 95%

N-Lauroylsarcosine sodium salt (95%) is a moderately pure anionic surfactant widely employed in molecular biology, protein biochemistry, micelle research, and oral hygiene studies. This amphiphilic compound facilitates the solubilization of membrane proteins and glycoproteins, including those difficult to isolate using harsher detergents like SDS. Its unique properties allow it to inhibit microbial flora and act as a fungistatic agent in aqueous systems.

This grade of N-lauroylsarcosine is suitable for general research, especially for cell lysis, protein separation, RNA extraction, and micellar behavior studies. Its utility spans applications in both biochemical and biophysical fields.

Key Features and Benefits

• High surfactant efficiency at ≥94% assay purity
• Mild membrane disruption without full denaturation
• Useful in RNA purification buffers with guanidinium salts
• Prevents foaming during high-salt lysis protocols
• Acts as a bacteriostatic/fungistatic agent at low concentrations
• Soluble in water (up to 293 g/L) for rapid reagent preparation
• Stable under dry conditions; hygroscopic and moisture-sensitive

Typical Applications

• Membrane Protein Solubilization
  Extracts integral membrane proteins and glycoproteins under mild conditions for structural studies or downstream analysis.
• Cell Lysis for RNA Purification
  Used in conjunction with guanidinium thiocyanate in phenol-free RNA isolation protocols to reduce protein contamination.
• Surfactant Behavior Studies
  Explored for its micellar properties and paramagnetic anisotropy in phase behavior analysis.
• Microbial Control in Oral & Gut Models
  Demonstrated inhibition of bacterial flora in vitro at concentrations as low as 0.25%.
• Fungistatic in Dispersions
  Functions as a microbial growth suppressant in aqueous systems at 1%.

Technical Specifications

References

• Gariglio P. et al., FEBS Letters, 1974, 44(3):330–333.
  (Sarkosyl activation of RNA polymerase in mitotic mouse cells)
• MacDonald R. J. et al., Methods in Enzymology, 1987, 152:219–227.
  (Isolation of RNA using guanidinium salts)

Thermo Scientific™ N-Lauroylsarcosine Sodium Salt (95%) is a versatile laboratory-grade surfactant designed for routine molecular biology and protein extraction. With a balance between performance and cost-effectiveness, this product is particularly suitable for protocols that do not require ultrapure reagents but demand reliable surfactant function and compatibility with nucleic acid and protein workflows.