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Invitrogen™ MitoTracker™ Dye for Mitochondria Labeling, 20 x 50 µg
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Specifications:
| Application | Flow Cytometry, Cell Analysis, Fluorescence-Based Assay | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Molecular Biology Reagent | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-performance rosamine- and carbocyanine-based fluorescent probes for live-cell mitochondrial staining and localization
Invitrogen™ MitoTracker™ dyes are cell-permeant, mitochondria-selective fluorescent probes designed for live-cell labeling and mitochondrial localization in fluorescence microscopy, flow cytometry, and microplate-based assays. These dyes accumulate in active mitochondria, providing robust and specific staining for mitochondrial visualization in multicolor imaging and cell analysis workflows.
MitoTracker dyes are available in rosamine-based and carbocyanine-based formats, with variants optimized for fixation-retention or live-cell only applications. The inclusion of a chloromethyl moiety in many MitoTracker dyes allows covalent binding to mitochondrial proteins, enabling staining retention after loss of mitochondrial membrane potential and during aldehyde fixation.
Available Dyes & Spectral Properties
| Catalog Number | Dye Name | Excitation/Emission | Type |
|---|---|---|---|
| M22425 | MitoTracker™ Red FM | 581/644 nm | Carbocyanine |
| M22426 | MitoTracker™ Deep Red FM | 644/665 nm | Carbocyanine |
| M7512 | MitoTracker™ Red CMXRos | 579/599 nm | Rosamine |
| M7513 | MitoTracker™ Red CM-H₂XRos | 579/599 nm | Rosamine (reduced) |
| M7514 | MitoTracker™ Green FM | 490/516 nm | Carbocyanine |
| M7510 | MitoTracker™ Orange CMTMRos | 554/576 nm | Rosamine |
| M7511 | MitoTracker™ Orange CM-H₂TMRos | 554/576 nm | Rosamine (reduced) |
Key Features
- Live-Cell Compatible – Passively diffuses across membranes, selectively staining active mitochondria
- Retention after Fixation – Many dyes remain bound post-fixation and permeabilization for downstream ICC or ISH
- Variety of Colors – Red, orange, green, and deep red options for multicolor labeling experiments
- Special Packaging – Supplied in 50 μg vials for fresh reconstitution, minimizing freeze–thaw degradation
- High Specificity – Accumulates in mitochondria based on membrane potential and/or covalent binding
- Flexible Detection – Compatible with fluorescence microscopy, flow cytometry, and plate readers
Applications
- Mitochondrial localization and morphology studies in live cells
- Cell cycle and apoptosis research involving mitochondrial dynamics
- Multicolor imaging with compatibility for green and red fluorophores
- Post-fixation assays such as immunocytochemistry and in situ hybridization
- Flow cytometry–based mitochondrial analysis
Specifications
| Parameter | Details |
|---|---|
| Detection Method | Fluorescence |
| For Use With | Fluorescence microscope, flow cytometer, microplate reader |
| Label Type | Fluorescent dye |
| Target | Mitochondria (organelle stain) |
| Format | 50 μg lyophilized vials (special packaging) |
| Storage Conditions | –5°C to –30°C, protect from light |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
MitoTracker™ dyes offer researchers a versatile, high-resolution method for labeling active mitochondria in live cells, with options for fixation retention and compatibility with multicolor imaging workflows. Their spectral variety, stability, and specificity make them an essential tool for mitochondrial biology, apoptosis research, and cell analysis.
- Description: MitoTracker™ Deep Red FM, 644/665 nm MitoTracker™ Red FM, 581/644 nm MitoTracker™ Red CMXRos, 579/599 nm MitoTracker™ Red CM-H2Xros, 579/599 nm MitoTracker™ Green FM, 490/516 nm MitoTracker™ Orange CMTMRos, 554/576 nm MitoTracker™ Orange CM-H2TMRos, 554/576 nm