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Thermo Scientific™ n-Nonyl-β-D-glucoside (NG), ≥99%
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Specifications:
| Application | Protein Biology, Protein crystallography | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₁₅H₃₀O₆ |
High-Purity Non-Ionic Detergent for Membrane Protein Solubilization and Stabilization
Thermo Scientific™ n-Nonyl-β-D-glucoside (NG) is a high-purity, non-ionic glucoside detergent designed for gentle and efficient solubilization of membrane proteins and hydrophobic biomolecules. Derived from glucose and featuring a nonyl (C9) alkyl chain, NG offers enhanced hydrophobicity and forms larger micelles compared to shorter-chain glucosides, making it particularly suitable for membrane protein extraction, purification, and structural studies.
Its biocompatibility, low UV absorbance, and high aqueous solubility make NG an ideal surfactant for use in NMR, cryo-EM, crystallography, and proteomics workflows where the preservation of native protein structure is essential.
Key Features & Benefits
- Gentle, Non-Ionic Detergent – Minimizes protein denaturation and preserves biological activity
- C9 Alkyl Chain – Enhances hydrophobic interactions for effective membrane protein solubilization
- Large Micelle Formation – Facilitates stabilization of large or multi-subunit protein complexes
- High Purity (≥99%) – HPLC-verified for consistency and minimal UV interference
- Water-Soluble & Biocompatible – Easily dissolved at cold temperatures; ideal for delicate protein systems
- Ready for Structural Applications – Suitable for cryo-EM, NMR, and crystallization assays
Applications
- Membrane Protein Extraction – Ideal for solubilizing GPCRs, ion channels, and transporters
- Protein Stabilization – Maintains structural integrity for functional and structural assays
- Structural Biology – Used in cryo-electron microscopy (cryo-EM), NMR spectroscopy, and X-ray crystallography
- Reconstitution Studies – Supports incorporation of proteins into liposomes or nanodiscs
- Biophysical Characterization – Enables dynamic light scattering (DLS), size-exclusion chromatography (SEC), and other techniques
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | n-Nonyl-β-D-glucoside (NG) |
| Catalog Numbers | A65516 (5 g), A65515 (1 g) |
| CAS Number | 69984-73-2 |
| Molecular Formula | C₁₅H₃₀O₆ |
| Molecular Weight | 308.44 g/mol |
| CMC (in H₂O) | ~6.5 mM (0.20% w/v) |
| Aggregation Number | ~133 |
| Purity | ≥99% (β + α, by HPLC) |
| Solubility | ≥20% in water (0°C to 5°C) |
| Form | Lyophilized powder |
| pH (1% Solution) | 5–8 |
| Absorbance at 280 nm | Low (suitable for UV-transparent workflows) |
| Storage Conditions | Store at –20°C in a dry, light-protected container |
| Regulatory Use | For Research Use Only. Not for use in diagnostic procedures. |
Thermo Scientific™ n-Nonyl-β-D-glucoside (NG) is a trusted non-ionic detergent for the gentle yet effective solubilization of membrane proteins, supporting a wide range of biochemical and structural biology workflows. Its high purity, large micelle size, and excellent compatibility with sensitive analytical techniques make it an essential reagent for researchers working with fragile or hydrophobic proteins.
- Pack Size: 1g 5g