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Thermo Scientific™ HEPES powder, Ultrapure
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Formula: C₈H₁₈N₂O₄S
Specifications:
| Application | molecular biology | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Alfa Aesar | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific's HEPES is an ultrapure, high-quality zwitterionic buffer widely used in biological and biochemical applications. Its superior purity (≥99%, dry basis) and precise buffering capabilities make it ideal for cell culture, enzymatic studies, and molecular biology experiments.
Chemical Overview
- Chemical Name: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulphonic acid
- Synonyms:
- HEPES free acid
- N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)
- 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
- N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid
- 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
- Molecular Formula: C₈H₁₈N₂O₄S
- Molecular Weight: 238.31 g/mol
- CAS Number: 7365-45-9
- EINECS Number: 230-907-9
- MDL Number: MFCD00006158
- Merck Index: 14,04654
Physical and Chemical Properties
| Property | Details |
|---|---|
| Purity (Assay) | ≥99% (dry basis) |
| Appearance | White crystalline powder |
| Melting Point | ~236 °C (decomposition) |
| Density | 1.448 g/cm³ (20 °C) |
| pKa (20 °C) | 7.55 ± 0.15 |
| UV Absorbance | ≤0.2 (at 250 nm, 1 M, 1 cm) |
| Loss on Drying | ≤0.5% |
| Residue on Ignition | ≤0.1% |
| Chloride (Cl) | ≤0.005% |
| Phosphate (PO₄) | ≤0.001% |
| Sulphate (SO₄) | ≤0.01% |
| Iron (Fe) | ≤5 ppm |
| Heavy Metals (Pb) | ≤10 ppm |
Applications
- Biological Buffers:
- Used to maintain stable pH in cell culture media and biological systems.
- Optimal for experiments that require pH stability in the range of 6.8 to 8.2.
- Biochemical Assays:
- Provides reliable pH control for enzymatic reactions.
- Commonly used in protein and nucleic acid research.
- Molecular Biology:
- Applied in buffer systems for electrophoresis and other analytical techniques.
- Cell Culture:
- Supports cellular processes during prolonged experiments outside CO₂ incubators.
Advantages
- High Purity: Meets stringent analytical standards (≥99% assay) to ensure reproducibility in experiments.
- Precise Buffering: Narrow pKa range (7.55 ± 0.15) ensures stable pH near physiological conditions.
- Low Contaminants: Minimal chloride, phosphate, sulphate, and heavy metals for sensitive applications.
- UV Transparency: Low absorbance at 250 nm ensures compatibility with optical applications.
Specifications
| Test Parameter | Specification |
|---|---|
| Assay (Purity) | ≥99% (dry basis) |
| pKa (20 °C) | 7.55 ± 0.15 |
| UV Absorbance (250 nm) | ≤0.2 |
| Loss on Drying | ≤0.5% |
| Residue on Ignition | ≤0.1% |
| Chloride (Cl) | ≤0.005% |
| Phosphate (PO₄) | ≤0.001% |
| Sulphate (SO₄) | ≤0.01% |
| Iron (Fe) | ≤5 ppm |
| Heavy Metals (Pb) | ≤10 ppm |
Storage and Handling
- Storage Temperature: Store at ambient temperature in a dry, ventilated area.
- Shelf Life: Stable under recommended conditions.
- Packaging: Supplied as a white crystalline powder for ease of handling.
Safety Information
- Hazard Classification: Irritant; avoid inhalation and skin contact.
- Precautions: Use gloves, goggles, and protective clothing when handling.
- Disposal: Dispose of in accordance with local chemical waste regulations.
Thermo Scientific HEPES Ultrapure Buffer is a versatile and reliable buffer for biological and biochemical research. Its high purity, chemical stability, and low UV absorbance make it suitable for critical applications in cell culture, molecular biology, and biochemical assays requiring consistent pH control.
- Pack Size: 1kg 100g 5kg