Gibco™ AlbuMAX™ II Lipid-Rich BSA

 Gibco™ AlbuMAX™ II Lipid-Rich BSA is a specialized, lipid-rich, bovine serum albumin (BSA) product designed for cell culture applications requiring high levels of lipid supplementation. It is commonly used in serum-free and low-serum media formulations to support cell growth, improve viability, and enhance protein production in mammalian cell cultures.

Key Features

1. Lipid-Rich Formulation:
   • Enriched with lipids, including cholesterol and fatty acids, to support cells with higher lipid metabolism demands.
2. High Purity:
   • Highly purified BSA with minimal impurities to ensure optimal cell performance.
3. Serum-Free Compatibility:
   • Ideal for serum-free and low-serum culture systems, minimizing variability caused by serum additives.
4. Improved Cell Performance:
   • Enhances growth and viability of cells, including hybridomas, CHO cells, and other mammalian cell lines.
5. Consistent Quality:
   • Manufactured under stringent quality controls to ensure batch-to-batch consistency.

Applications

1. Cell Culture:
   • Provides essential lipids and nutrients for mammalian cell lines in serum-free or low-serum environments.
2. Hybridoma Growth:
   • Supports the production of monoclonal antibodies by hybridoma cell lines.
3. Protein Expression:
   • Enhances recombinant protein production in CHO and HEK293 cell lines.
4. Virus Production:
   • Used in virus production protocols requiring serum-free culture conditions.
5. Cryopreservation:
   • Improves cell survival during freezing and thawing processes when added to cryopreservation media.

Specifications

• Type: Lipid-rich bovine serum albumin (BSA)
• Form: Powder
• Purity: ≥99% BSA with added lipids
• Solubility: Readily soluble in aqueous media
• pH: Neutral in solution
• Storage Conditions: Store at 2–8°C in a dry, tightly sealed container.

Preparation and Usage

1. Dissolution:
   • Dissolve in sterile water or culture medium to the desired concentration, typically between 0.1% and 5% (w/v).
2. Filtration:
   • Sterile filter the solution after preparation for use in aseptic environments.
3. Incorporation:
   • Add directly to serum-free or low-serum media formulations as a supplement.

Benefits

1. Serum Replacement:
   • Reduces reliance on fetal bovine serum (FBS), providing a more defined culture environment.
2. Lipid Supplementation:
   • Supports cell lines with high lipid requirements, enhancing growth and productivity.
3. Batch-to-Batch Consistency:
   • Manufactured to minimize variability, ensuring reproducibility in research and production.

Safety and Handling

• For Research Use Only: Not intended for human or animal therapeutic use.
• Protective Measures: Use gloves, lab coats, and eye protection when handling the product.
• Disposal: Follow local regulations for laboratory waste disposal.

Gibco™ AlbuMAX™ II Lipid-Rich BSA is a reliable and versatile supplement for advanced cell culture systems, providing essential lipids and nutrients to support growth and productivity in diverse mammalian cell lines.

Comparison of Gibco™ AlbuMAX™ I and AlbuMAX™ II

AlbuMAX™ I and AlbuMAX™ II are lipid-rich bovine serum albumin (BSA) products from Gibco™, designed for use in serum-free and low-serum cell culture applications. While they share similar core functions, they differ in lipid content, specific applications, and optimization for cell types.

Key Differences

Key Similarities

1. Lipid-Rich BSA:
   • Both products are lipid-enriched to support cell metabolism and growth in serum-free or low-serum conditions.
2. High Purity:
   • Both are highly purified, with minimal impurities, ensuring reproducibility in experimental workflows.
3. Applications:
   • Both are effective in cell culture, protein expression, hybridoma growth, and cryopreservation.
4. Batch Consistency:
   • Both products are manufactured under strict quality controls for consistent performance.
5. Format:
   • Supplied as powders, easily soluble in aqueous solutions.

Usage Recommendations

• Choose AlbuMAX™ I:
  • For general cell culture applications requiring moderate lipid supplementation.
  • When cost efficiency is important in routine experiments.
• Choose AlbuMAX™ II:
  • For specialized or high-performance applications, such as monoclonal antibody production or high-demand recombinant protein expression.
  • For cell lines with significant lipid metabolic requirements.

Storage and Handling

• Storage Conditions:
  • Both products should be stored at 2–8°C in a tightly sealed container.
• Preparation:
  • Both require reconstitution in sterile water or media, followed by sterile filtration.

Summary

• AlbuMAX™ I is suitable for less demanding cell culture systems, providing sufficient lipid supplementation for general applications.
• AlbuMAX™ II offers superior lipid content and is optimized for advanced, high-yield applications in biotechnology and pharmaceutical production.