Thermo Scientific™ GeneRuler 50 bp DNA Ladder with 6X TriTrack DNA Loading Dye
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Specifications:
Application | Protein Electrophoresis & Western Blotting | ||
Storage Temperature | 2-8°C | ||
Product Type | DNA Ladders | Forms | Liquid |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
The Thermo Scientific™ GeneRuler 50 bp DNA Ladder is a reliable, chromatography-purified double-stranded DNA molecular weight marker ideal for sizing and approximate quantification of DNA fragments from 50 bp to 1,000 bp. It consists of 13 precisely defined DNA fragments and is supplied with a 6X TriTrack DNA Loading Dye, allowing three-color tracking during electrophoresis.
This DNA ladder is designed for use in agarose or polyacrylamide gels and can be visualized with ethidium bromide or SYBR Safe. Its consistent band intensity and reference bands enable accurate fragment sizing and semi-quantitative analysis of sample DNA.
Key Features
- Broad size range: 50 bp to 1,000 bp
- 13 DNA fragments for comprehensive sizing
- Defined DNA quantities per band for approximate quantification
- Sharp, well-defined bands for easy visualization and analysis
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Supplied with 6X TriTrack DNA Loading Dye, containing:
- Xylene Cyanol FF
- Bromophenol Blue
- Orange G
- Three-color migration tracking during electrophoresis
- Compatible with 1–2% agarose gels and standard gel staining agents
Band Sizes
50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000 bp
(Note: Certain bands may be more intense for orientation and reference.)
Applications
- Sizing and quantification of PCR products
- DNA fragment analysis for cloning or restriction digestion
- Routine gel electrophoresis in teaching and research labs
- Verification of synthetic or isolated DNA fragments
Specifications
Property | Value |
---|---|
Product Type | DNA Ladder (double-stranded) |
Size Range | 50 bp to 1,000 bp |
Number of Fragments | 13 |
Concentration | 50 μg per vial |
Loading Dye | 6X TriTrack (3-color) |
Ready to Load | No (loading dye provided separately) |
Gel Compatibility | Agarose and polyacrylamide |
Detection Methods | Ethidium bromide, SYBR Safe |
Label or Dye | Xylene Cyanol FF, Bromophenol Blue, Orange G |
Green Features | Sustainable packaging |
Product Line | GeneRuler |
Unit Size | 50 µg (SM0371), 5 × 50 µg (SM0372) |
Storage Conditions | –20°C (avoid repeated freeze-thaw cycles) |
Intended Use | For Research Use Only. Not for diagnostic use. |
Available Formats
Catalog Number | Quantity |
---|---|
SM0371 | 50 µg |
SM0372 | 5 × 50 µg (bulk pack) |
The Thermo Scientific™ GeneRuler 50 bp DNA Ladder provides high-resolution separation and visualization for short DNA fragments, making it ideal for PCR product verification, fragment sizing, and molecular cloning workflows. With its consistent band intensity and 13 distinct fragments, this ladder offers accurate sizing, three-color tracking, and trusted performance across a wide range of applications in molecular biology.
- Pack Size: 50 µg with 1 mL Loading dye for 100 Applications 5 x 50 µg with 2 x 1 mL Loading dye for 500 Applications